Santhosh V, Gomathi D. M, Khadeja-Bi A, Suganya S, Gurulakshmi G, Manjula D. N. Study of Serum Electrolytes in Type 2 Diabetes Mellitus Individuals in Rural Tertiary Care Hospital in Kancheepuram District. Biomed Pharmacol J 2021;14(2).

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Manuscript received on :03-12-2020
Manuscript accepted on :17-04-2021
Published online on: 24-04-2021

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Plagiarism Check: Yes
Reviewed by: Dr. B.Surendiran  
Second Review by: Dr. Salman Ahmed Pharmacognosy  
Final Approval by: Dr Ayush Dogra

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V. Santhosh¹, D M. Gomathi¹, A. Khadeja Bi¹, S. Suganya¹, G. Gurulakshmi² and Manjula Devi N³

¹Department of Biochemistry, Karpaga Vinayaga Institute of Medical Science and Research Center.

²Government Medical college,Virudhunagar.

³Department of Biostatistician, Karpaga Vinayaga Institute of Medical Science and Research Center.

Corresponding Author E-mail: gomathisurendran01@gmail.com

DOI : https://dx.doi.org/10.13005/bpj/2171

Abstract

Aim: To measure the levels of  Serum electrolytes (Na+, K+ ,Cl )in type II DM individuals and to compare it with healthy controls. Methods: A case control study was conducted at Medicine Department, from March 2019 to September 2019. A total of 60 patients ,comprised of  30 confirmed type II DM patients as cases and  30  healthy individuals of similar criteria were treated as controls. In both the groups,biochemical measurement of Serum electrolytes (Na+, K+ ,Cl ),FBS was studied and the results were compared. Results: Inindividuals with Diabetes mellitus sodium and chloride showed insignificant alterations.There was an increase in serum potassium levels which was found  to be statistically highly significant (p-value less than or equal to 0.05) . The drift of  potassium from  intracellular space to extracellular space leads to Hyperkalaemia which is due torenal impairment, insulin deficiency or hypertonicity. Conclusion: This study concludes that there is significant association of  potassium  with hyperglycemic crisis in patients with  type 2 diabetes mellitus. Thus serum electrolytes has to be routinely monitored in diabetic individuals since electrolyte derangements are markedly found in uncontrolled diabetes.

Keywords

Diabetes Mellitus; Hyperkalaemia; Hyperglycemic

Introduction

Diabetes Mellitus is one among the most common metabolic abnormality .More than 62 million individuals are being diagnosed with the disease and it is gaining the status of potential epidemic in India ¹.

Altered distribution of electrolytes leads to electrolyte imbalance in patients suffering from diabetes which is due to osmotic fluid shifts  induced by hyperglycaemia that is  caused by osmotic diuresis. The potassium intake of cells remains normal whereas there is impairment in the insulin mediated glucose intake ².

The redistribution of potassium from the space present intercellularly to space present extracellularly in  type II DM patients leads to increase in tonicity of plasma that causes Hyperkalaemia. Dysnatremias are caused by diabetes by means of several underlying mechanisms. One among them is osmotically active substance glucose. Movement of water out of the cells occurs  due to increase in osmolality of serum which is caused by hyperglycaemia. Hypovolemic hyponatremia as a result of osmotic diuresis is also caused by uncontrolled diabetes mellitus. The electrolyte loss in urine will exacerbate the renal sodium wasting in diabetic ketoacidosis ²

Controlling the electrical gradient  of body fluids, nerve conduction , blood clotting,muscle contraction as well as acid base balance are carried over by electrolytes which are chemical compounds present in the body fluids. Enzyme activities and intermediatory metabolism are being carried over by  major macro minerals like Sodium ,Potassium , Chloride and Calcium. Derangements  in  the serum electrolyte levels are  related  with  Diabetes Mellitus. Electrolyte derangement that is occurring as a result of complications of diabetes are fatal in severe form and thus requires immediate and urgent management ¹. Hence the study was done to assess the levels of serum electrolytes among type II diabetic individuals in comparison with  healthy controls.

Aims and Objectives

To measure the Serum electrolytes levels of Sodium (Na+),Potassium(K+ ) and Chloride(Cl ) in type II DM individuals.

Materials and Methods

This study includes 30 type II DM individuals as cases and  30  healthy individuals of similar criteria were treated as controls from  Department of Medicine, KIMS& RC.

 Methodology

Study design : Case control study

Study setting: Department of Biochemistry and General Medicine,KIMS& RC.

Study duration: March 2019 -September 2019

Study population: Patients attending OP and IP in age group of 40 to 60 years in Department of Medicine .

Sample size and sampling:60 patients

Inclusion & Exclusion criteria:

Inclusion criteria: Individuals with Type 2 Diabetes mellitus.

Exclusion criteria: Individuals with thyroid dysfunction,metabolic syndrome

Study instruments: Biochemical analysis of Fasting Blood Glucose  by enzymatic method in fully automated analyser, Electrolytes( sodium, potassium and chloride) by spot -chem  electrolyte analyser.

Data collection: Written consent from patients, Fasting and electrolyte samples as per SOP.

Data analysis: The data was analyzed by SPSS software.

Results

This study includes 60 individuals in which 30 were cases(diabetic) and 30 were controls(non-diabetic)  with matched age and sex.

Table 1

Above table includes Sodium, potassium and chloride levels in both cases and control groups in which mean of diabetic individuals sodium levels were found to be less compared with non-diabetic individuals but it is not statistically significant (P>0.05).

Figure 1: Comparison between mean values of electrolytes between DM and Non-DM Individuals. Click here to view figure

It was found that the mean of  chloride and potassium levels was slightly higher when compared with controls in which chloride is not statistically significant (P>0.05) and potassium is found statistically significant (p<0.05).

Discussion

The association between serum electrolytes and blood glucose involves multiple factors that includes age and other associated conditions ³. Hyperglycaemia in   diabetes results in shifting of water from the  intracellularto extracellular space  thereby diluting the  sodium present extracellularly leading  to lowered serum sodium levels ⁴. Changes in renin angiotensin system in diabetes alsoleads to alteration in serum sodium concentration.In this study there is no statistical significance in the levels of serum sodium.

Hypovolemic – hyponatremia  as a result of  osmotic diuresis is caused due to uncontrolled diabetes mellitus. Osmotic diuresis leads to loss of water as well as solutes ⁵. Subsequent changes in  sodium levels present  in the serum and osmolarity  of serum has  a strong association between  solutes and water. Hypernatremia can  also occur in diabetic patients that results due to loss of hypotonic fluid in urine which in turn occurs because of osmoticdiuretic sequelae of  glycosuria ⁶.

The drift of  potassium toextracellular space leads to Hyperkalaemiawhich is due  to renal impairment, insulin deficiency or hypertonicity. In this study  hyperkalaemia is noted in diabetes patients and is significant compared to healthy individuals.

The cellular uptake of potassium is found to be normal whereas there is impairment in the insulin-mediated absorption of glucose. This situation is homogenous with divergence of intracellular pathways that follows insulin receptor activation ⁷.

Intracellular dehydration leads to efflux of potassium from the cell that occurs as result of osmotically induced movement of water transcellularly which acts as desirable gradient for potassium efflux ⁸. Insulin deficiency, rhabdomyolysis, hypertonicity and acidosis are examples of shift induced hyperkalaemia in type 2 diabetic individuals. Chronic kidney disease causing decreased glomerular filtration in potassium levels as well as many drugs like renin-inhibitors, angiotensin-converting enzyme inhibitors ,beta blockers, potassium-sparing diuretics interfering potassium excretion are associated with Hyperkalaemia..The syndrome of hyporeninaemic hypoaldosteronism  leads to decreased tubular secretion of potassium which is one of the common cause for chronic hyperkalaemia. There is  high risk for hyperkalaemia in elderly diabetic individuals who are  on drugs that are known to interfere with  homeostasis of potassium, hence careful monitoring of potassium levels are required in such individuals ⁹.

Elevated chloride level occurs in type 2 diabetic individuals occurs as result of  diabetic ketoacidosis. Decrease in pH of blood is caused by ketoacidswhich in turn disturbs the acid base balance resulting in increased chloride levels. In this study there is no statistical significance in the chloride levels.

In our study, we found statistical significant difference in potassium levels between the controls and diabetic group. Hyperkalaemia was evident in the diabetic group. This finding was also seen in study conducted by Datchinamoorthi, et al ¹ which proved that diabetic individuals were more prone to  increased levels of chloride and hyperkalaemia, Sodium and individuals were more prone to  increased levels of chloride and hyperkalaemia.,Sodium and chloride showed no statistical significance in this study. Ogunleye, et al. inhis study in 2016have shown that diabetic individuals had decreased levels of  calcium, sulphur, magnesium, phosphorous,potassium,sodium and chloride  as compared to non-diabetic individuals. Loss of these electrolytes results due to increased excretion of these electrolytes in urineor  as result of decreased absorption, which in turn leads todeficiency of elements or marginal states of elements in diabetic individuals ¹⁰

Conclusion

This study concludes that there is significant association of  potassium  with hyperglycaemic crisis in type 2 diabetic individuals.  It is also concluded that for monitoring the prognosis of type II DM individuals,the assessment of electrolyte related derangements are very helpful ^{11, 12, 13}. It is also observedthat  the risk of diabetic complications may be as a result of  significant role of  electrolyte imbalance. Thus serum electrolytes has to be routinely monitored in diabetic individualssince electrolyte derangements are markedly found in uncontrolled diabetes ^{14, 15}.

References

1. V. R. R. B. Datchinamoorthi Sarguru, “Evaluation of Serum Electrolytes in Type II Diabetes Mellitus,” Int. J. Pharm. Sci. Rev. Res., pp. 40(1) : 251-253, 2016.
2. B. S. Das Alaka, “Evaluation of Serum Electrolyte Levels in Type 2 Diabetes Mellitus.,” Indian Journal of Applied Research, vol. 6, no. 8, pp. 91-93., 2016.
3. K. S. ..T. R. .. Jiskani S. A, “Disturbances in Serum Electrolytes in Type 2 Diabetes Mellitus,” National Journal of Health Sciences, vol. 3 , no. 4, pp. 128-131., 2018.
   CrossRef
4. E.. Hasona N .A, “Evaluation of Electrolytes Imbalance and Dyslipidaemia in Diabetic Patients.,” Med. Sci., vol. 4, no. 7, 2016.
   CrossRef
5. L. E. B. F. E. M. Liamis G, “Diabetes mellitus and electrolyte disorders,” vol. 2 , no. 10, 2014.
   CrossRef
6. T. A. K. K. S. K. Elisaf MS, “ Acid-base and electrolyte disturbances in patients with diabetic ketoacidosis,” Diabetes Res Clin Pract , vol. 34, pp. 23-27., 1996.
   CrossRef
7. R. B. E. Hillier TA, “Hyponatremia: Evaluating the correction factor for hyperglycaemia.,” Am J Med 1999, vol. 106, 399-403 .
   CrossRef
8. G. D. .Shoback, “New York: McGraw-Hill Medical.,” Greenspan’s basic & clinical endocrinology (9th ed.)., 2011.
9. M. a. D. J. C. Biff F. Palmer, “Electrolyte and Acid–Base Disturbances in Patients with Diabetes Mellitus,” n engl j med., 2015.
10. K. M. Virtanen SM, “Nutritional risk predictors of beta cell autoimmunity and type 1 diabetes at a young age”.,” The American. Journal of Clinical Nutrition, vol. 78, no. 6, p. 1053–67, .
    CrossRef
11. P. R. Joshi SR, “India-diabetes capital of the World:now heading towards hypertension,” J Assoc. Physicians.., vol. 55, pp. 323-4, 2007.
12. G. M. R. P. V. Kumar A, “India towards diabetes Control;key issues,” Australas Med J, vol. 6, no. 10, pp. 524-31, 2013 .
    CrossRef
13. W. S. e. al., “Global prevelance of Diabetes;,” Diabetic Care. , vol. 27, no. 5, pp. 1047-1053, 2004 ;.
    CrossRef
14. F. Husain, M. Arif Maan, M. Sheikh, H. Nawaz and A. Jamil, “ Trace elements status in type 2 diabetes.,” Bangladesh J. Med. Sci., vol. 8, p. 52–56., 2009, .
    CrossRef
15. Kitabchi, G. Umpierre, M. Murphy and R. Kriesberg, “Hyperglycaemic crisis in adult patients with diabetes: A consensus statement from the American diabetes,” Diabetes Care , 29, p. 2739–2748., 2006.
    CrossRef