Teware K, Singh P, Mehta R. Phytochemical Extraction and Analysis of Medicinally Important Plant Cissus qudrangularis L. (Hadjod). Biomed Pharmacol J 2011;4(1)

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Manuscript received on :April 09, 2011
Manuscript accepted on :May 19, 2011
Published online on: 27-11-2015

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Kalpana Teware¹, Pratibha Singh² and Rakesh Mehta³

¹Department of Bio-Science SAM Girls P.G. College, Bhopal India.

²Government S.N.College, Bhopal India.

³Government MGM. College Itarsi India.

Abstract

Cissus qudrangularis L. (Hadjod) belongs to Vitaceae family is an indigenous medicinal plant of India. It has been prescribed in ancient Ayurvedic texts by Bhava Prakash and Chakra Dutta as a general tonic especially for the fractured patient. The stem of Cissus quadrangular is L. is also reputed in Ayurveda as alterative, anthelmintic, dyspeptic, digestive, tonic, analgesic in eye and ear diseases, in the treatment of irregular menstruation and asthma, and in complaints of the back and spine. The plant extracts also exhibit cardio tonic property. in present study there are various test reveals the presence of Alkaloids ,Carbohydrates, Glycosides Tannins – phenolic compounds Protein and amino acids Gum and mucilage Flavones and flavonoids Saponins Steroids and sterols, in the various types of extract as Petroleum ether extract ,Chloroform extract ,Ethyl Acetate Extract, n-Butanol. Preliminary qualitative chemical tests of extracts were found positive results for Phytosterol, flavonoids and triterpenoids in Ethyl acetate fraction, Hydro alcoholic fraction was positive to carbohydrate, tannin and amino acid and vitamin C. Flavonoids and triterpenoids are the active constituents in stem of Cissus quadrangularis L. and may be responsible for its pharmacological activities The results of the study could be useful in setting some diagnostic indices for the identification and preparation of a monograph of the plant.

Keywords

Cissus quadrangularisL; flavones; fluorescence; saponins; alkaloids

Introduction

Nature has provided a complete store-house of remedies to cure all aliments of mankind This is where, nature provides us drugs in the form of herbs, plants and algae’s to cure the incurable diseases without any toxic effect .Cissus qudrangularis L.(Hadjod) belongs to vitaceae family is an indigenous medicinal plant of India. The use of this plant by the common folk for promoting fracture healing process is an old practice. It has been prescribed in ancient Ayurvedic texts by Bhava Prakash and Chakra Dutta as a general tonic especially for the fractured patient. Since then it has been in extensive use by bone setters both for external application and as an internal medicine to be taken with milk. The stem of Cissus qudrangularis L.is also reputed in Ayurveda as alterative, anthelmintic, dyspeptic, digestive, tonic, analgesic in eye and ear diseases, in the treatment of irregular menstruation and asthma, and in complaints of the back and spine. Scientific studies have revealed the Cissus extract to possess cardiotonic and androgenic property.¹The plant extracts also exhibit cardiotonic property.²The Cissus quadrangularis Linn. Has been recognized as a rich source of carotenoids, triterpenoids and ascorbic acid and is proved to have potential for medical effects, including “Gastro protective activity” in conjugation with NSAID therapy and in “Lipid metabolism and oxidative stress”. The Cissus quadrangularis L. plant contains high amount of vitamin C, Carotene A, anabolic steroidal substances and calcium. Stem contains two asymmetric tetra cyclic triterpenoids; Onocer-7-ene-3b-21a diol & Onocer-7ene 3a, 2,1 diol.2, 3, 4The present research focuses on the extraction as well as evaluation of the different phytochemical present in this.

Material and Methods

Collection of the plant

The plant was collected from MFP park (SANJEEVANI) Barkheda Pathani, Bhopal India.

Drying and size reduction

After identification and authentication stems were subjected to drying in normal environmental condition under shade with some change in temperature in oven. The dried stems were powdered by pulverization and were stored in air tight container

Fluorescence Characteristic of the Different Extracts of the Stems of Cissus quadrangularis L.

The fluorescence characteristic of different extracts was studied by observing them under

UV Light at 365nm. The tests and observations are recorded in the table below.

Table 1: Fluorescence Characteristic Of The Different Extracts of The Stems of Cissus quadrangularis L.

Extracti

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