eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2016-12-22 9 3 919 925 10.13005/bpj/1030 10594 article Huali Yang 1 Na Liu 1 Shaochin Lee 1 School of Life Sciences, Shanxi University, Taiyuan, Shanxi, PR China. The study investigated the underlying molecular mechanisms of ethanol extract of Annona muricata. L induced liver cancer cell apoptosis. The anti-proliferative effect of ethanol extract of Annona muricata. L was evaluated by MTT assay. Flow cytometry was used to detect the cell cycle and apoptosis. TUNEL assay was used to confirm cell apoptosis. DCFH-DA fluorescent probe can detect the level of intracellular reactive oxygen species (ROS). Different concentrations of ethanol extract of Annona muricata. L leaves (0, 30, 60, 120, 240 µg/ml) were used to treat liver cancer HepG2 cells for 24h or 48h, the cell viability was inhibited in time- and dose-dependent manners. Ethanol extract of Annona muricata. L leaves increased the number of cells in the sub-G1 fraction in a dose-dependent manner. TUNEL assay confirmed that the extract caused apoptosis of the cancer cells. Further analysis revealed that the extract produced reactive oxygen species (ROS). Antioxidant N-acetylcysteine was able to inhibit the extract-triggered ROS production and apoptosis. In conclusion, our results suggest that the ethanol extract of Annona muricata. L leaves induces HepG2 cell apoptosis through ROS pathway. https://biomedpharmajournal.org/vol9no3/ethanol-extract-of-annona-muricata-l-induces-liver-cancer-cell-apoptosis-through-ros-pathway/ Annona muricata. L liver cancer cell apoptosis ROS