eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2016-12-22 9 3 1101 1105 10.13005/bpj/1055 11834 article Ramachandran Tamilselvi 1 Rajendran Mathan Rajan 2 Veronica Aruna Kumari 3 Deivanayagam Kandaswamy 4 Department of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospital, Bharath University, Chennai, Tamilnadu, India. Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences Sri Ramachandra University, Chennai, Tamilnadu, India. Department of Conservative Dentistry and Endodontics, Madha Dental College and Hospital, Kundrathur, Chennai, Tamilnadu, India. The study was performed to find the influence of organic matrix on hardness and fracture toughness of the human dentin. The mid-sagittal sections of the dentin were subjected to Vickers hardness testing after removal of organic component from dentin by heat treatment. The heat treatment temperature for human dentin was determined as 800°C by thermogravimetric analysis in order to facilitate complete removal of organic component. The removal of proteins from dentin was confirmed by Fourier transform infrared spectroscopy. The significant difference in hardness and fracture toughness values was found among the heat treated and untreated dentin (p<0.001).  This study confirms the role of organic component of dentin on   mechanical properties. Thus, helps in research and development of novel reparative materials. https://biomedpharmajournal.org/vol9no3/efficacy-of-organic-component-on-hardness-and-fracture-toughness-of-human-dentin-using-heat-treatment-in-vitro-study/ Human dentin collagen hardness fracture toughness thermo gravimetric analysis Fourier transform infrared spectroscopy heat treatment