eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2016-08-21 9 2 519 524 10.13005/bpj/967 7529 article Tran Thi Hien 1,5 Do Thi Ha 3 Do Minh Truong 2 Loi Vu Duc 4 Trong Tuan Dao 3 Nguyen Thanh Hai 4 Department of pharmacy, Thai Binh medical and pharmacy University, Vietnam. College of Sciences, Thai Nguyen University, Vietnam. National Institute of Medicinal Materials, 3B Quangtrung, Hanoi, Vietnam. School of Medicine and Pharmacy, Hanoi National University, 144 Xuan Thuy street, Cau Giay, Hanoi, Vietnam. Faculty of medicine, Lund University, Sweden. p53, a tumor suppressor protein, has important roles in DNA repair, cell cycle and apoptosis, is a one of the key events in cancer development. Coix lacryma-jobi seed has been used as a food and traditional medicine plant with anti-oxidant, anti-cancer and anti-diabetic effects. In currently research, we identified the most potent p53-increasing compound among 4 compounds (1 – 4) found in Coix lacryma-jobi and demonstrated its molecular mechanism in MCF-7 cells. Among the four isolated compounds (1 – 4), triolein most increased p53. Triolein treatment induced p53, p21, p27 and Bax in MCF-7 cells. Moreover, triolein caused S phase arrest through suppression of CDK1, phopho-Rb and E2F1 in dose-dependent manner. We also observed the decreasing of DNA synthesis by triolein. These data suggest that triolein may induced cell cycle restart involve DNA synthesis and apoptosis pathway in MCF-7 cells. https://biomedpharmajournal.org/vol9no2/triolein-from-coix-lacryma-jobi-induces-cell-cycle-arrest-through-p53p21-signaling-pathway/ Triolein  Coix lacryma-jobi  MCF-7 cells apoptosis DNA synthesis