eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2016-08-21 9 2 433 441 10.13005/bpj/956 7259 article I. W. G. Gunawan 1 A. A. Bawa Putra 2 I. A. G. Widihati 3 Laboratory of Organic Chemistry, Department of Chemistry, Udayana of University. Laboratory of Anorganik Chemistry, Department of Chemistry, Udayana University. Laboratory of Physical chemistry, Department of Chemistry, Udayana of University, Bali-Indonesia. Increased antioxidant activity of α-humulene of Hibiscus manihot L leaf empirically may help prevent oxidative stress due to the production of compounds reactive oxygen species (ROS), thereby protecting cells from damage function. Oxidative stress can be formed through an increase in the activity of the compound 8-hydroxy-2-deoksiguanosin in the body. This study is a laboratory experimental design was randomized pre and post-test control group design. A total of 36 wistar rats were divided into 4 groups, namely P₀ (negative control), group P₁ (isolate α-humulene dose of 5 mg/kg, P₂ (isolate α-humulene dose of 10 mg/kg), and P₃ (isolate α-humlene dose of 15 mg/kg ). All groups induced by alloxan dose of 140 mg/kg in order to get the condition. After the treatment for 12 weeks, serum was taken for examination of 8-OHdG levels and see a picture immunohistochemistry pancreatic tissue of wistar rats. Data were analyzed by one-way ANOVA and continued test t-paired to determine the mean difference between treatments with significance limit of 5%. the results showed that, the dose α-humulene isolate of Hibiscus manihot L resulted in a decrease in the average of 8-hydroxy-2-deoksiguanosin levels in the positive control group was (3.63 ± 0, 19) ng / mL, the treatment groups respectively P₁ by (3.94 ± 0.51) ng/mL; P₂ was (3.49 ± 0.47) ng/mL and P₃ of (1.99 ± 0,18) ng/mL. It is also evident from the results of immunohistochemical against 8-OHdG profile repair due to damage pancreatic β-cells in the islets of Langerhans. This difference was statistically significant was shown with a value of p<0.05. Therefore α-humulene compounds Hibiscus manihot L leaf may help prevent oxidative stress through reduction mechanism 8-OHdG. https://biomedpharmajournal.org/vol9no2/the-response-to-oxidative-stress-%ce%b1-humulene-compounds-hibiscus-manihot-l-leaf-on-the-activity-of-8-hydroxy-2-deoksiquanosin-levels-pancreatic-%ce%b2-cells-in-diabetic-rats/ Oxidative Stress α-humulene Hibiscus manihot L 8-OHdG Diabetes