Manuscript accepted on :August 05, 2016
Published online on: --
N. Balachander, E. Rajesh, S. Hari Priya and K. M. K. Masthan
Department of Oral Pathology, Sree Balaji Dental College and Hospital, Bharath University, Pallikaranai, Chennai-600100.
DOI : https://dx.doi.org/10.13005/bpj/1013
Abstract
The pathology studies widely deal with many cellular and nuclear altered structures other than these one of the important and interesting features is the observation of various histopathological bodies. These inclusion bodies is an important diagnostic-aid in identifying the underlying disease. Therefore in this article present different inclusion bodies seen in various diseases.
Keywords
histopathologica; diagnostic-aid ; pathology
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Introduction
Inclusion bodies are nuclear or cytoplasmic aggregates which are stainable substances, usually proteins, and formed due to viral multiplication or genetic disorders in human beings these bodies are either intracellular or extracellular abnormalities and they are specific to certain diseases. When a foreign gene or the infectious agent is injected into a cell, the complementary DNA translated from a messenger RNA may code for a protein, which are fails to undergo further modification, transport, condensation of the cell, result in inclusion body. In some diseased conditions, cells modified and may become pathognomonic for that particular disease.
Classification Of Inclusion Bodies1
Physiological inclusion bodies
Infection inclusion bodies
Inclusion bodies in viral condition
Intra cytoplasmic inclusions
Intra nuclear inclusions
Inclusion Bodies seen in bacterial infections.
Inclusion Bodies seen in fungal infections.
Inclusion bodies in neoplasms.
Inclusion bodies in autoimmune diseases.
Inclusion bodies seen in blood dyscrasias.
Inclusion bodies seen in cystic lesions.
Physiological inclusion bodies
Odland bodies
In Keratinized stratified squamous epithelium is shows membrane-coating granules called Odland bodies. It is also called as lamellar bodies, keratinosomes. These are seen in the upper stratum spinosum and stratum granular cell layers which are rich in glycolipids. These lipids are discharged extracellularly to form a permeability barrier that prevent absorption of aqueous fluids.[2]
Weibel Palade bodies
They are Storage granules of endothelial cells, von Willebrand factor and P-selectin are two principal molecules are stored in the bodies released when needed. Thus its play important role in haemostasis and inflammation. Infection inclusion bodies.[3]
Infection inclusion bodies
nclusion bodies in viral condition
Intra cytoplasmic inclusions
Councilman Bodies[6]
Described → they were named by an American Pathologist William T. Councilman as they called by his name ‘Councilman Bodies’.
Type →acidophilic inclusion bodies
Morphology → cytoplasm of hepatocytes, with ballooning degeneration. This is due to hepatocytes undergoing apoptosis.
Diseases → viral infections such as viral hepatitis and yellow fever.
Henderson Peterson bodies[7]
Type → intracytoplasmic inclusions
Diseases → Molluscum Contagiosum disease caused by Pox viruses spinous and corneum of the infected epithelium
Morphology → large ellipsoid, homogenous, which are nuclear and cytoplasmic aggregates bodies.
Intra nuclear inclusions
Cowdry Type- A[8,9]
Diseases → gingivostomatitis and conjunctivitis caused by herpes simplex and also chicken pox caused by varicella zoster. These bodies
Morphology → acidophilic material of droplet-like masses surrounded by clear halos within nuclei.
Lipschutz bodies[10]
Type → eosinophilic nuclear inclusions
Morphology → enlarged nuclei and clear halo
Diseases → varicella zoster and herpes simplex
Cowdry Type- B[11]
Type → intranuclear eosinophilic without any nuclear change
Morphology → amorphous or droplet like bodies surrounded by clear halo in diseases like amorphous or droplet like bodies surrounded by clear halo infection.
‘Owl’s eye’[12]
Type → they are large intranuclear viral inclusion bodies with thickened nuclear membrane
Morphology → appear owl’s eye
Diseases → Hodgkin’s lymphomas
Inclusion Bodies seen in bacterial infections.
Dohle bodies[4,5]
Morphology → light blue-grey, oval, basophilic staining areas in the cytoplasm of neutrophils shows defect in maturation of neutrophils.
Diseases → typhoid, diphtheria,and tuberculosis.
Stain → Leishman-Giemsa stain and Romanowsky stain.
Inclusion Bodies seen in fungal infections.
Asteroid bodies[13,14]
Morphology → stellate shape with numerous rays radiating from the central core.
Diseases → Sporotrichosis
‘Toto bodies
Morphology → homogeneous, eosinophilic pools of material seen in superficial spinous layer of the surface epithelium
Diseases → epulis fissuratum.
Inclusion bodies in neoplasm.
Wagner- Meissner body[15,16]
Morphology → oval aggregates of eosinophilic globules containing parallel slits
Diseases → von Recklinghausen’s disease of skin, neurofibroma
Verocay bodies
Morphology → arranged spindle shaped cells with a palisading pattern
Diseases → benign nerve sheath tumor, Schwannoma
Psammoma bodies[17,18]
Morphology → spherical, concentrically laminated mass of calcified material these bodies are formed due to necrosis followed by dystrophic calcification.
Diseases → numerous benign and malignant epithelial and connective tissue tumors such as psammomatoid meningioma, psammomatoid juvenile ossifying fibroma, psammomatoid melanotic schwannoma, cystadenocarcinoma.
Stain → H&E stain
Russell bodies
Described → Michaels (1935)
Diseases → chronic inflammatory granulomata, multiple myeloma, plasmacytoma, helicobacter pylori infection, periapical granuloma.
Stain → GrunwaldGiemsa stain, fibrin, periodic acid Schiff
Pustulo- Ovoid bodies[19-21]
Type → eosinophilic inclusions
Morphology → round by aggregation coalescing granules
Diseases → granular cell tumors
Kamino bodies
Type → eosinophilic inclusion bodies
Morphology → globules
Diseases → pigmented spindle cell nevus, Spitz nevus
Stain → trichrome stains and periodic Acid- Schiff’s
Dutcher bodies
Described → Dutcher and Fahey
Type → intranuclear inclusions
Morphology → smooth, membrane-bound and surrounded by clumped chromatin, immunoglobulin protein.
Diseases → chronic synovitisandlarge B- cell lymphoma and multiple myeloma.
Stain → Wright- Giemsa stains and periodic acidSchiff’s
Inclusion Bodies in autoimmune diseases.[1]
Civatte bodies
Type→ eosinophilic
Morphology → waverly arranged fine filaments entangledwith desmosomes, melanosomes, and other organelles.
Diseases→ discoid lupus erythematosus and lichen planus
Formation→due to basal cell liquefaction degeneration and hypergranulosis
Derivation→ basal cells and connective tissue elements from the basement membrane zone
Stain→ for keratin
Hematoxylin bodies
Type→ basophilic extracellular aggregation
Morphology→ ovoid in shape, necrotic loci and contain densechromatin
Diseases→ systemic lupus erythematous
‘Schaumann bodies
Described→ Jorge Schaumann in 1941
Morphology→ large concentrically lamellated structure seen in the cytoplasm of the giant cells, presence of calcium and phosphorus and small quantities of iron in Schaumann bodies
Diseases→ Sarcoidosis, tuberculosis, hypersensitive pneumonitis
These inclusion bodies enlarge within the and giant cells and macrophages later rupture the cytoplasmic membrane of cell appear in extra cellular matrix excluded crystals underwent further deposition of minerals leading to extracellular calcifications.
Inclusion bodies seen in blood dyscrasias.[1]
Heinz bodies
Described→ Robert Heinz in 1890
Morphology→ irregular, small, deep purple granules in red blood corpuscles
Diseases→ Glucose-6-phosphate dehydrogenase deficiency, haemolytic anemias, hemolytic anemias
Stain→ Wright’s stain and crystal violet
Formation→ oxidative damage of DNA or change in aminoacids morphology in RBC
Howell- Jolly bodies
Described → William Henry Howell and Justin Marie Jolly
Morphology→ dark staining small round inclusions and ring like appearance in the red blood corpuscles – mimics parasites
It is presented as remnant of DNA during its maturation in bone marrow
Diseases→ Pernicious anemia and Leukaemia with megaloblastic anemia
Inclusion bodies seen in cystic lesions[1]
Rushton bodies/ Hyaline bodies
Two Types→ eosinophilic granular core and concentrically lamellatedsome hyaline bodies are partly lamellar and partly granular
Morphology→ various shape linear, curved, hairpin shaped, straight, circular or polycyclic forms.Mostly seen in the epithelial lining and rarelyin fibrous capsule
Ultrastructure→ two forms- lamellated and homogeneous is composed of outermost electron dense and electron lucent layers
Stain→ Mallory aldehyde fuchsin, periodic acid-Schiff, Gomori stains, Papanicolaou and Orcein.
Diseases→ Plexiform Ameloblastoma, Residual Cyst and Radicular Cyst.
Conclusion
Disease progression occurs with biochemical and cellular changes. Presence of inclusion bodies indicates disease. Absence of them indicates the disease subsidence. Inclusion bodies in the course of the disease at various stages is used in staging the diseases and for their treatment planning.
Reference
- Histopathologic bodies: An insight, Meena Kulkarni, Tripti Agrawal, Varsha Dhas Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, DPU Pimpri, Pune, India.
- Svane-Knudsen V. Stratum corneum barrier lipids in cholesteatoma. Acta Otolaryngol Suppl 2000;12:139-42.
- Lowenstein CJ, Morrell CN, Yamakuchi M. Regulation of weibelpalade body exocytosis. Trends Cardiovasc 2005;15:302-8.
- Easton JA, Fessas C. The incidence of dohle bodies in various diseases and their association with thrombocytopenia. Br J Haematol 1966;12:54-60.
- Weiner W, Topley E. Dohle bodies in the leucocytes of patientswith burns. J Clin Pathol 1955;8:324-8.
- Available from: www.htt p://microblog.me.uk/167. [Last accessedon 2012 Oct 15].
- Sangster A. Non-glandular theory of origin of Molluscum contegiousum. Lecture on skin diseases at Charing cross Hospital;1880.
- Available from: htt p://www.mondofacto.com. [Last accessed on2012 Oct 15].
- Eyons AS, Kaslow RA. Viral Infections of Humans: Epidemiologyand Control. British Journal of Biomedical Science. June 1, 1998;1029;813.
- Antonio C, Slootweg PJ. Pathology of the head and neck; VesiculobullousDiseases, 1999;72.
- Available from: http://www.humpath.com/cowdry-type-Bnuclear-inclusions [Last accessed on 2012 Feb 8].
- Matt es FM, McLaughlin JE, Emery VC, Clark DA, Griffi ths PD.Histopathological detection of owl’s eye inclusions is still specificfor cytomegalovirus in the era of human herpesviruses 6 and 7.Clin Pathol 2000;53:612-4.
- Rajendran R, Shivapathsundharam B. Shafer’s Textbook Of OralPathology. 6th ed. Mycotic Infections of Oral Cavity. p. 369.
- Berson SD, Brandt FA. Primary pulmonary sporotrichosis withunusual fungal morphology. Thorax 1977;32:505-8.
- Ben ZP. Head and neck surgical pathology; Soft Tissue Pathologyof Head and Neck. 1997. p. 397-419.
- . Ohno J, Iwahashi T, Ozasa R, Okamura K, Taniguchi K. Solitaryneurofi broma of the gingival with prominent diff erentiation ofMeissener bodies: A case report. Diagn Pathol 2010;5:61.
- Keating RF, Goodrich JT, Packer RJ, et al. Tumors of the pediatriccetral nervous system; Chapter 25; Intracranial meningioma. p. 356.
- Enzinger FM, Weiss SW, et al. Soft Tissue Tumours – 3rd ed. Chapter31; Benign Tumors of Peripheral Nerves; p. 860-1.
- Simansky DA, Aviel-Ronen S, Reder I, Paley M, Refaely Y, YellinA. Psammomatous melanotic schwannoma: Presentation of a rarelung tumor. Ann Thorac Surg 2000;70:671-2.
- Sorbe B, Frankendal B. Prognostic importance of Psammoma bodies in adenocarcinomas of the ovaries. Gynecol Oncol1982;14:6-14.
- Solomon MC, Khandelwal S, Raghu A, Carnelio S. PsammomatoidJuvenile Ossifying Fibroma of the Mandible- A Histochemicalinsight. Internet J Dent Sci 2009;7.