eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2016-04-28 9 1 293 298 10.13005/bpj/938 6483 article Said Al-Dalaen 1 Jihad Alzyoud 2 Aiman Al-Qtaitat 3 Department of Pharmacology, Faculty of Medicine, Mutah University, Karak, Jordan Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Hashimite University, Zarqa, Jordan Department of Anatomy and Histology, Faculty of Medicine, Mutah University, Karak, Jordan Arginine is an amino acid, aid in liver detoxication. Carbon tetrachloride (CCl₄) induces hepatic injury by the initiation of lipid peroxidation process through the formation of free radicals, leading to liver damage. Objective: The present work aimed to investigate whether the administration of arginine had protective and/or curative effects against hepatic lipid peroxidation induced by CCl₄ in rats.Sixty Swiss albino rats included in the present study were subdivided into four groups equally: (1) control group, (2) CCl_4-intoxicated group; rats were injected intraperitoneally (i.p.) with CCl₄, (3) protection group; rats were pre-treated with arginine for 6 days, then injected i.p. with CCl₄ and (4) curative group; rats were injected i.p. with CCl₄, 24 hours later, rats were post-treated with arginine for six days. Rats were sacrificed at the end of the treatment and liver tissues were obtained, homogenized and used for biochemical analyses. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase, glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S- transferase (GST) and reduced glutathione (GSH) levels were assayed by spectrophotometry.CCl₄ intoxication increased the level of hepatic MDA, decreased the level of hepatic GSH and inhibited the activity of antioxidant enzymes (GPx, GR, GST, SOD and catalase) versus their levels in the normal group. Pre- and post-treatment with arginine decreased hepatic level of MDA, increased GSH level and improved the activities of all antioxidant enzymes compared to the untreated CCl₄–intoxicated rats. Arginine administration have hepetoprotective and hepatocurative effects against CCl₄ induced lipid peroxidation, oxidative stress and liver damage, also, the curative effects of arginine were found to be more effective than its protective effects. https://biomedpharmajournal.org/vol9no1/the-effects-of-l-arginine-in-modulating-liver-antioxidant-biomarkers-within-carbon-tetrachloride-induced-hepatotoxicity-experimental-study-in-rats/ Arginine Lipid peroxidation Antioxidant enzymes Liver damage Carbon tetrachloride (CCl₄)