eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2016-04-28 9 1 215 228 10.13005/bpj/929 6911 article Bahareh Hashemi 1 Azim Akbarzadeh 2 Mehdi Arjmand 3 Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran Professor in Clinical Biochemistry, Pasteur Institute of Iran Associated professor in Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran Today, nanotechnology has been imported in various fields of agriculture, pharmacy, medicine, electronics and food industries. Due to existence of the increasing need to develop safe, non-toxic and environmentally friendly methods (green nanotechnology), the wide range of nanostructures are made todays with the purpose of increasing the amount of healthy, non-toxicity and being environmentally friendly.Nanotechnology science and its products have found a good position in the pharmaceutical area, so that the global pharmaceutical market witnesses numerous pharmaceutical products using nanotechnology and many drugs such as drug delivering nanostructures are designed for less concentrated and more effective transferring of the drug and to reduce side effects as well as to enhance efficiency of treatment, that the chemotherapy drugs are some of them. So, the aim of this study was to provide formulation of nanoarchaeosomal paclitaxel and to evaluate parameters such as encapsulation, particle sizeand size distribution. To prepare archaeosome, the first phase was cultivation of Halobacterium salinarum and extraction of archaeosome from it. Then, it has been combined a certain proportion of cholesterol, archaeosome and paclitaxel with each other and the average diameter of the nanoarchaeosomal paclitaxel was measured with the aid of Zeta Sizer that was equal to 425 nm. The encapsulation efficiency of nanoarchaeosomal paclitaxel was obtained %96.32. Cell viability was examined by MTT assay on MCF-7 cell lines. Analysis on Cell viability percentage showed that dead effect of tumor cells in formulation of nanoarchaeosomal paclitaxel was more than free formulation. It means that the nanoarchaeosomal paclitaxel was more effective than paclitaxel in a freeform. https://biomedpharmajournal.org/vol9no1/providing-the-formulation-of-nanoarchaeosomal-paclitaxel-and-evaluating-parameters-of-encapsulation-particle-size-and-size-distribution/ Nanotechnology Breast cancer MCF-7 cell line Archaeosome nano carrier Paclitaxel