eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-12-28 8 2 1363 1368 10.13005/bpj/896 5983 article Ramin Abri 1 Taghi Zahraei Salehi 1 Vadood Razavilar 2 Mohammad Ahangarzadeh Rezaee 3 Afshin Javadi 4 Department of Microbiology, Science and Research branch, Islamic Azad University, Tehran, Iran. Department of Food Hygiene, Science and Research branch, Islamic Azad University, Tehran, Iran. Infectious Diseases and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Iran. Department of Food Hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran To determine the virulence markers of enterotoxigenic E. coli [ETEC] and enteropathogenic E. coli [EPEC], 224 stool specimens from diarrheic humans and 60 diarrheic calves were examined by PCR. This study was conducted to achieve a better understanding of the genetic relationships and evolutionary patterns within E. coli pathotypes isolated from humans and calves. Stool specimens from 224 diarrheic humans and 60 diarrheic calves were cultured for E. coli. The samples were obtained from North West of Iran. The rep-PCR fingerprint technique, which performed with primer BOX A1R, was used for discriminating between human and calf sources. A total of 25 ETEC and EPEC strains isolated from humans and calves. The discriminant analysis showed an Average Rate of Correct Classification [ARCC] of 97% for ETEC and 94.5% for EPEC isolates. This result reveals that the rep-PCR fingerprint technique with primer BOX A1R may be an effective technique for discriminating and grouping E. coli isolates, and can be empolyed as a source following tool for differentiation and identification of host sources. https://biomedpharmajournal.org/vol8no2/differentiation-of-enterotoxigenic-and-enteropathogenic-escherichia-coli-isolated-from-humans-and-calves-by-rep-pcr/ ETEC EPEC Human Calves rep-PCR