eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-12-28 8 2 547 55 10.13005/bpj/797 3186 article I Made Sukadana 1 Sri Rahayu Santi 1 Chemistry Department Faculty of Mathematic and Natural Sciences Udayana University Gayam seed (Inocarpus fagiferus Fosb) is empirically has an ability to reduce oxidative stress diseases such as atherosclerosis. This study aims to prove the antioxidant compounds such as linoleic acid, ethyl linoleic, ethyl oleic, and homopterocarpine in ethanol extract of gayam seed (Inocarpus fagiferus Fosb) to prevent atherosclerosis through increase SOD-3 expression and decrease of MDA concentration plasma blood of Wistar rat with high cholesterol diet for 16 weeks. This was an experimental study with randomized posttest only control group design. The samples were 25 Wistar rat, randomized into 5 treatment groups, i.e.  group P₀ (negative control), group P₁ (positive control, feed high cholesterol diet), group P₂ (high cholesterol diet and ethanol extract in dose of 50 mg/kg bw), group P₃ (high cholesterol diet and ethanol extract in dose of 100 mg/kg bw), and group P₄ (high cholesterol diet and ethanol extract in dose of 150 mg/kg bw). After 16 weeks treatment, blood of the rats were driven for MDA and all rats were then euthanasia to obtain their aorta for immunohistochemistry analyzed to give expression of SOD-3 data. All of data was analyzed by Anova to obtain the treatment different toward control by statistically with significance at a =0.05. The results showed that ethanol extracts of gayam seed (Inocarpus fagiferus Fosb) in doses of 50-150 mg/kg bw increase the expression of positive SOD-3 aorta endhotel cell and decrease the concentration of  MDA significantly (p<0.05). The expression of positive SOD-3 aorta endhotel cell is marker endogenous antioxidant, therefore, the antioxidant compounds in ethanol extract of gayam seed can to prevent atherosclerosis disease through of SOD inducer mechanism. https://biomedpharmajournal.org/vol8no2/antioxidant-compounds-of-gayam-seed-inocarpus-fagiferus-fosb-to-prevent-atherosclerosis-in-wistar-rat-with-high-cholesterol-diet/ Inocarpus fagiferus Fosb antioxidants SOD-3 MDA atherosclerosis