eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-06-18 8 1 503 512 10.13005/bpj/640 1584 article Shadi Akhavan 1 Mahnaz Qomi 1 Gayaneh Charmahali 2 Sharareh Akhavan 2 Department of Medicinal Chemistry (Pharmaceutical Sciences Research Center), Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. Department of applied chemistry Islamic Azad University , Shahr-e-rey Branch, shahr-e-rey, Iran Zopiclone is used to treat sleeping problems (insomnia). In the current work, for the first time, a three-phase hollow-fiber liquid-phase microextraction combined with HPLC-UV was proposed for the determination of zopiclone in urine samples. Different factors that can affect the extraction process such as extraction solvent, acceptor and donor phase composition, salt addition, stirring rate, extraction time and extraction temperature were optimized. Under the optimum conditions, detection and quantitation limits obtained 53.9 and 161.7ng mL^-1, respectively, and enrichment factors 116 were obtained. The calibration curves were linear within the range of 161.7-2000 ng mL^-1 with estimation of coefficient higher than 0.9990. Within a day and between day RSDs were 5.51% and 5.34%, respectively. Finally, the proposed method was applied to the detection and determination of zopiclone in human urine sample. https://biomedpharmajournal.org/vol8no1/hollow-ifber-based-liquid-phase-microextraction-combined-high-performance-liquid-chromatograph-for-the-determination-trace-amounts-of-opiclone-in-biological-fluids/ Zopiclone High performance liquid chromatography Hollow fiber based liquid phase microextraction Microextraction