eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-05-03 7 2 715 725 10.13005/bpj/546 3279 article Seyedeh Neda Miraee 1 Mahnaz Qomi 1 Forough Shamshiri 2 Parviz Raoufi 3 Department of Medicinal Chemistry (Pharmaceutical Sciences Research Center) Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran Methylphenidate hydrochloride is used fortreatment ofhyperactivity disorder. In the current work, for the first time a microextraction technique was introduced to detection and quantification of methylphenidate hydrochloride in urine and plasma samples. Hollow ûber based liquid phase microextraction (HF-LPME) followed by high performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection was used for extraction of methylphenidate hydrochloride. The organic membrane solventconsists of 1-Octanol immobilized in the pores of a hollow fiber. A pH gradient was driving force to migrate analyte from sample solution, through the organic liquid membrane into an acidic acceptor solution which was located inside the lumen of hollow fiber.Extraction recoveries upper than 80% were obtainedin different biological matrices which resulted inpreconcentration factors upper than 112 and acceptable repeatability (2.4< RSD% <4.8). The method offers good linearity with estimation of coefficient higher than 0.9990. Finally, it was applied to the determination and quantification of methylphenidate hydrochloride in biological samples. https://biomedpharmajournal.org/vol7no2/hollow-fiber-liquid-phase-microextraction-followed-by-high-performance-liquid-chromatography-for-the-determination-of-trace-amounts-of-methylphenidate-hydrochloride-in-biological-fluids/ Methylphenidate hydrochloride High performance liquid chromatography Hollow fiber based liquid phase microextraction Microextraction.