eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-05-02 7 1 129 135 10.13005/bpj/462 2872 article Padera Faryadyan 1 Afra Khosravi 2 Meysam Kashiri 3 Reza Valizadeh 4 Psychosocial Injuries Research Centre, Ilam University of Medical Sciences, Ilam, IIran. Department of Immunology, Faculty of Medicine, Ilam University of Medical Sciences. Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran. Department of Psychology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran. There are many studies indicating that prenatal condition influences offspring different behaviors. In this study we investigated the effect of prenatal exposure to different colors on variations of serum testosterone levels in adult male offspring rats. Four groups of pregnant female Wistar rats, seven rats in each group, were enrolled in this study. The pregnant rats were placed in color chambers including green, blue, black and white (control). After delivery, male offspring of all color groups transferred to clear Plexiglas’s chambers until age 8-9 weeks and their serum testosterone concentration were measured using the ELISA Kit.The serum testosterone level was significantly high in adult male offspring’s who were prenatally exposed to blue and green color compared to male offspring’s who were prenatally exposed to black and white colors. Increasing of serum testosterone levels was also significant in adult male rats who were prenatally exposed to blue color. The results showed serious changes of testosterone in male offspring’s due to prenatal exposure to different colors which can be translated as testosterone variables. We can document that stimulation during pregnancy can lead to testosterone changes in male offspring and such changes can be made by colors. https://biomedpharmajournal.org/vol7no1/prenatal-exposure-to-blue-and-green-colors-increases-serum-testosterone-levels-in-male-offspring-rats/ Testosterone Prenatal Male offspring Color Rat ElISA Kit