eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-04-26 5 2 391 393 2568 article Yasmin C. Attar 1 Sayali Randive 1 Sadiccha Patil 1 Mrudula M. Bendigeri 1 Department of Microbiology, Rajaram College, Vidyanagar, Kolhapur - 416 004, India. The biosurfactant producing Acinetobacter sp. YMP which showed vegetable oil degradation potential on MSM was studied for lipase production. The production of lipase was confirmed on fat agar & lipase activity was measured by titrimetric method. The effect of environmental factors (agitation, time span , substrate concentration , pH & temperature ) on lipase production was studied by using Acinetobacter sp. YMP. The enzyme was further purified by Ammonium sulphate (60%) precipitation technique. The maximum lipase production by Acinetobacter sp. YMP was found to be within 120 hours at 450 rpm, 3.0 pH , 27° C temperature & at 2% substrate concentration. This species was also able to show upto 8 % oil degradation potential on MSM broth & agar. https://biomedpharmajournal.org/vol5no2/study-of-lipase-production-by-acinetobacter-sp-ymp/ Biosurfactant MSM (Mineral salt medium) Lipase