eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-03-28 4 1 95 105 1734 article Suresh Kumar Karri 1 S.Srinivasan 2 Department of Pharmacology, Annamalai University, Chidambaram India. Department of Pharmaceutics, Krupanidhi College of Pharmacy, Bangalore - 560 035 India. The present study was aimed to investigate the single dose effects of a herbomineral amino acid extract (HMAA) of Spinacia oleracea on alloxan induced diabetic nephropathy rats. In the present study, the animals were divided into 6 groups Group 1 – Normal saline as vehicle –control, Group 2 – Untreated diabetic rats (Alloxan induced), Group 3 – Animals treated with Metformin (0.5 mg/kg) – standard drug, Group 4 – Animals treated with Extract alone, Group 5 – Animals treated with Herbal + Mineral, Group 6 – Animals treated with Herbal + Mineral + Amino Acid. Based on the oxidative stress hypothesis of alloxan action, the role of herbal extract on free radicals in the pathology of diabetes mellitus has been studied in diabetic nephropathy rats. The present study demonstrated that herbal extract in the presence of an aminoacid (L-Arginine, 0.1 mM) and mineral (Zn+, in the form of Zinc Sulphate, 0.5 mM) could be a potent antioxidant and further, the data suggested that the mechanism underlying such protection is mediated via restoration of diabetic nephropathy leading to normalization of the diabetic rats. https://biomedpharmajournal.org/vol4no1/effect-of-herbomineral-amino-acid-formulation-on-wistar-strain-diabetic-nephropathy-rats/ Spinacia oleracea HMAA anti-oxidant diabetic nephropathy