Zare H, Mohsenzade S, Mobaien F. The Effect of Allium Sativum on Blood Biochemical Factors in People with High Blood Cholesterol and Diabetes. Biomed Pharmacol J 2010;3(2)

---

Manuscript received on :June 25, 2010
Manuscript accepted on :August 12, 2010
Published online on: 24-11-2015

---

How to Citeclose    |   Publication History close

 Views: 

Visited 3,361 times, 1 visit(s) today

 

 PDF Downloads: 

1549

Habibeh Zare¹, Sasan Mohsenzade² and F. Mobaien³

¹Department of Biology, Payame Noor University, Busher Iran.

²Department of Biology, Shiraz University Iran.

³Bushehr Medical University Iran.

Abstract

Introduction Two important dangerous factors of atrosclerose disease are diabetes mellitus and cholesterolemia . from the old time garlic was was used and several studies were accomplished about the effects of garlic on decreasing blood sugar and blood cholesterol that some of them proved this subject and some of them. rejected that the objective of this research in the study of the effect of allium sativum consumption on some of blood biochemical factors in persons with high cholesterol and diabetes. Method This clinical study was accomplished on 25 persons with necessary qualifications and with more than 126 mg in deciliter blood sugar and 245 mg/dl blood cholesterol . each volunteer used 5 gr allium sativum twice in a day for 42 days and then did not use allium sativum at all for 42 days .3 stages of phlebotomy was done as fasting in order to measuring the biochemical factors. The results were analyzed by using ANOVA test and p<0.05 was considered meaningful. Results The average amount of fasting blood sugar (p<0.01) and total cholesterol (p<0.001) after 42 days allium sativum consumption decreased meaningfully. But after stopping allium sativum consumption it increased again.HDL-C increased meaningfully HbALC decreased(p<0.05).allium sativum consumption did not show meaningful effect on ceratinin urine of uric acid of cholesterol liver function tests with low. Conclusion With regard to current research results, it can be concluded that allium sativum consumption can decrease total cholesterol of blood ,FBS and HbALC and can increase HDL-C so garlic can be useful in light diabetes mellitus and hyper lipidemic especially in persons that can not bear chemical medicines.

Keywords

Hyper lipidemic; blood sugar increasing

Introduction

Artery sclerose   or Artero sclerose   related   to internal layer of blood-vessels that various factors have role in creating and progressing of it. Two factor of these are: diabetes mellitus and hyper lipidemic that by carrying it, one can prevent many indispositions of artery scleros. Diabetes  is one the oldest disease that is recognized and its global occurring increase significantly in current decades that in addition to creating artery sclerose ,it has other chronic indispositions, including.

And non blood –vessel indispositions . in most of countries ,enormous expenditures  spent for currying diabetes and hyper lipid yearly. Using herbaceous medicines begin to increase with attention to oldness of herbaceous medicine in iran,law expenditure of its preparation with regard to chemical  medicines and also very little indispositions of its consumption as compare with chemical medicines .garlic was used for different purposes (as food and medicinal) since thousands years ago being that of its important remedial features ,one can point to decreasing blood pressure  , anti cancer ,healing up wounds and decreasing blood sugar . many researches are done about the decrescent effects  of blood fatness and heart –vessel disease as the result of garlic consumption .some time results were contradictory in these researches .allium sativum effects on blood’s lipids are attributed to control of liver enzymes that are responsible for cholesterol synthesis and the decrescent  effect of blood sugar are know as the result of increasing of insulin excretion from since the most of done studies were on animals and in none of them allium sativum used for research ,so in this research the effect of allium sativum consumption on decreasing blood sugar ,cholesterol and other blood biochemical factors in persons with high blood sugar and cholesterol was considered.

Method

In this chemical study, 25 persons(18 women and 7 men) of those who called on rafsanjan specialized pathology laboratory of medical university for requested tests of rafsanjan physicians and had below features selected for doing study. total cholesterol of more than 245 mg/dl the age of lower than 60 years old with the average(45+/- 2.14) non-consumption of any effective medicine on blood sugar and cholesterol donot having certain diet propensity to cooperation till the end of project non consumption of garlic for 7 previous weeks at least. With attention to this that only allium sativum was used in this research that is consumption is current in society therefore there is not any ethical problem with regard to consumption material. Necessary explanation was given to all the volunteers about the project and the manner of doing the study and if it was agreed the composed questioner was complited. Before consuming allium sativum , in fasting condition , the blood sample was provided and blood sugar, total cholesterol,HDL-C,LDL-C. triglyceride ,urine, ceratinine , uric acid ,phosphate alkaline ,AST factors were measured by enzyme and total bilirobin method and hemoglobin glycos  by chemical method   in medical  laboratory. Size and weight and blood pressure of persons were measured and recorded in questionnaire .volunteers consumed sgr packet allium sativum twice a day (in company with diner and lunch )for 42 days.after 42 day consumption of allium sativum phlebotomy was done for the second time in fasting  state and also size and weight and blood pressure were measured  again. After this stage studied persons did not consume allium sativum at all for 42 days and then mentioned tests were done for third times in fasting state and size and weight and blood pressure were measured and recorded. After doing tests and obtaining results data analyzed by SPSS software. For comparing blood factors ANOVA test was used in 3 stages of phlebotomy and in the event that the difference between averages were meaningful ,Toki-Keramer couple test for comparing the groups was used and p<0.05 was considered as meaningful level.

Results

25 persons that had tatal cholesterol of more than 245 mg/dl and fasting blood sugar of more than 126 mg/dl were considered in this study. Allium stavium consumption by these persons lead to decrease of fasting blood sugar in 42 days with(p<0.01) that at the end of study and by no consuming of allium stavium , it increases again. Also tatal  cholesterol amount decreased at the end of 42 days of allium stavium consumption (p<0.001),HDL-C amount increased during the study as at the end of 84 days had meaningful increase relative to the beginning of study(p<0.005).HbALC decreased after 84 days of beginning of study(p<0.05).others biochemical factors of blood did kkh have any meaningful difference as the result of allium stavium consumption.

Discussion

It is observed in this research that allium stavium consumption for 42 days has these effects on serum lipids: tatal cholesterol level after 42 days consumption of 10 gr allium stavium decreased with p<0.001 meaningful level.HDL-C amount showed increasing after 84 days at the end of study (p<0.05).about triglyceride although decrease was observed after 84 days,it was not meaningful statistically .also LDL-C amount did not have any meaningful difference with attention to decrease after 42 day of allium stavium consumption . in Dr Rahmani study,cholesterol level and LDL-C decreased by recommending garlic powder for 12 weeks.the study of kanar and his colleagues   showed decreasing of cholesterol and LDL-C and LDL-C after 12 weeks consumption of allium stavium . in a research that was done by Mour kous , the consumption of garlic food complementary and fish oil for one month leads to decreasing of cholesterol ,triglyceride ,LDL-C and increasing of HDL-C . in accordance to Bertold and his colleagues study garlic oil consumption for 4 weeks did not have any effect on lipid and lipoproteins amount.also in super kouniz study garlic powder consumption for 3 months did not have any effect on lipoproteins of HDL-C and LDL-C.in slonig  and his colleagues study on rats that were under low cholesterol diet, garlic consumption leaded to decrease of LDL-C cholesterol .with attention to mentioned researches and their comparison with the results of this study. It can be propounded that the results of the most of them are similar to our study and the difference of some of the studies can be related to kind and time of study because in this study unlike previous studies fresh allium stavium was used while in previous project powder with water extract or oily extract was used.in this study , allium stavium cause decreasing of fasting blood sugar of diabetic persons after 42 days consumption (p<0.01) and decreasing of hemoglobin glycose ,at the end of 84 days consumption with p<0.05 meaningful level was observed . in previous studies any research on diabetic humans were not observed but there are researches about diabetic animals.in shila and his colleagues study on diabetic rats,the consumption of one of the main components of allium stavium named.

Caused decreasing of  blood sugar . also in Anour research in 2003 the recommending of garlic oil for 15 days in studies rats leaded to decreasing of blood sugar. The study of Ahmad and his colleagues showed the decrease of blood sugar in laboratory rats with regard to the consumption of allium stavium with ginger for 4 weeks.

Other biochemical factors

In this research , the effect of allium stavium consumption on other biochemical factors was not meaningful. But in the study of Anour that in addition to blood sugar and lipids, measured the amount of serum enzymes like phosphate alkaline , phosphate acid and 6 phosphate liver enzymes , had meaningful decrease . the study of ahmad showed the decrease of phosphate alkaline .it seems that the contradiction of this results with this study refers to the kind of study because above results were obtained in animals while  our study is on human. With attention to obtained results in this research .it can be propounded that allium stavium cause decreasing of serum sugar and lipids. although the decrease that has remedial role require using of other remedial methods. Certainly , there are specified benefits in using standardized medicines for diabetes and hyperlipidemic but secondary indispositions of these medicines had limited the use of these medicines in some persons. Therefore inpatients that have the increase of blood sugar , and fatness and can not bear chemical medicines allium stavium can be effective and safe method.

Acknowledgements

It is necessary to give thanks to all of the patients and colleagues that help us in this project.

References

1. Roberts S, Robergs R.fundamental principles of exercise physiology for fitness,performance and health. 428-429 (2000).
2. Eisenberg DM,David RB,Ettner SL,Apple S,Wilkey S,Van Rompay M,et al. trends in alternative medicine use in united states , 1990-1997: results of a follow-up national survey .JAMA, 280(18): 1569-75 (1998).
3. Hoerger TJ,Bala  MV,Bray  JW,Wilcosky  TC,LaRosa j.Treatment patterns and distribution of low-density lipoprotein cholesterol leves in treatment –eligible united states adults.Am J cardiol, 82(1): 61-5 (1998).
4. Maleki M, Oraii S .Cardiovascular disease in: Azizi F, Janghorbani M , and Hatami H . Epidemology and control of common disorders in iran, Endocrine and metabolism research
5. Fauci AS.Harrisons’s principle of internal medicine .16thed.New York:Mc Grow-Hill (2005).
6. Yeh YY and Liu L.cholesterol-lowering effect of garlic extracts and organosulfur compounds:Hamand and animal studies. Journal of Nitrition. 131: 989-993 (2001)
7. Bertold HK Sudhop T,Vonbergmann K. Effect of garlic oil preparation on serum lipoproteins and cholesterol metabolism .Arando mized controlledtrial.JAMA.279(23) (1998).
8. Superko HR,Krauss RM Garlic powder,effect of plasma lipids,postprandial lipemia, low –density lipoprotein particle size,high –density lipoprotein subclass distribution and lipoprotein .JAM Cell Cardiol. 35(2): 321-326 (2000).
9. Sheela CG Augustu KT. Antidiabetic effects of S-ally cysteine sulfoxide isolated from garlic allium stavium linn.indian J EXP Biol. 30(6): 523-526 (1992).
10. Patumraj S,Tewit S,Amatyakul S,Jariyapongskul A, Maneesri S,Kasantikul V, et al. Comparative effects of garlic and aspirin on diabetic cardiovascular complications. Drag Deliv., 7(2): 91-96 (2000).
11. Rahmani M.Effect of dried garlic supplementation on blood lipids in mild and moderate hypercholwstrolemic patients.Original article., 21-25 (1998).
12. Kannar D,Wattanapenpaiboon N,Saving GS,Wahliquist ML.Hypocholesterolemic effect on enterix-coated gfarlic supplement.j Am coll Nutr. 20(3): 225-231 (2001).
13. Marcus NC.Modulation of lipid profile by fish oil and garlic combination.J Nut L Med Assoc. 89(10): 673-678 (1997).
14. Slowing K,Ganado P,Sanz M,Ruiz E,Tetrina T.study of garlic extracts and fractions and cholesterol plasma level and vascular reactively in cholesterol fed rats.J Nutr. 131: 994-999 (2001).
15. Anvar MM,Mek AR.Oxidative stress in induced rat effects of garlic oil and metabolism .Biochem physiol. 135(4): 539-547 (2003).
16. Ahmed RS,Sharma SB.Biochemical studies on combined effects of garlic and ginger in albino rats.indian JEXP Biol. 35(8): 841-843 (1997).