eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-02-15 2 1 01 06 603 article A. Hasmah 1 Azimahtol Hawariah L. P. 2 Judith Hohmann 3 School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, (Malaysia) School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, (Malaysia) Institute of Pharmacognosy, University of Szeged, (Hungary) Cytochrome c is a central protein that plays a role in triggering apoptosis in cell induced by any agent either drug or plant extracts. Here, we present data of an active antiproliferative agent 7,3’,5’-trihydroxyflavanone (3HFD) that is elucidated from the plant Hydnophytum formicarium showed to trigger cytochrome c release in treated MCF-7 cell. By performing Western Blot, cytochrome c was seen to be elevated throughout the experiments. The increase level of cytochrome c was confirmed by ELISA and remarked as the beginning of the caspase cascade without altering caspase-8 level. In conclusion, the 3HFD seems to significantly induced apoptosis via mitochondria pathway as reflected by up regulation of cytochrome c. https://biomedpharmajournal.org/vol2no1/release-of-cytochrome-c-in-mcf-7-cells-treated-with-7-3-5-trihydroxyflavanone-of-hydnophytum-formicarium/ Cytochrome c MCF-7 cels Hydnophytum formicarium