eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-01-22 1 2 387 393 465 article V. Praveen Kumar 1 K. Shrinivasulu 2 P. Kalpana 3 Lecturer, Department of Biotechnology, K.L College of Engineering, Green Fields, Vaddeswaram, Guntur District (India). Lecturer, Department of Biotechnology, K.L College of Engineering, Green Fields, Vaddeswaram, Guntur District (India). Department of Biotechnology, K.L College of Engineering, Green Fields, Vaddeswaram, Guntur District (India). Production of alkaline protease employing the laboratory isolate, Bacillus sp. under solid state fermentation (SSF) was optimized. The effect of wheat bran was examined. The appropriate Incubation time, Temperature, pH, Moisture content, Inoculums size, Surfactant and mutagen effect were determined. Maximum yields of enzyme were achieved by employing wheat bran as substrates in 0.1 M carbonate/bicarbonate buffer at pH 10 with 1:1.5 moisture content at 48 hrs. Inoculums size and buffer solution level were found to be 30% for wheat bran. The increased growth of bacterium of mutagenic activity is seen with acrylamide solution. https://biomedpharmajournal.org/vol1no2/partial-purification-and-optimization-of-alkaline-protease-from-bacterial-ncim-2724-species-by-solid-state-fermentation/ Alkaline protease SSF NCIM