eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-01-22 1 2 445 448 505 article C.H. Madhusudan Reddy 1 G. Mubeen 1 Mamta Pal 1 Al-Ameen College of Pharmacy, Bangalore - 560 027 (India). HPTLC method for estimation of metformin HCl in single and in combination dosage form was developed and validated by using mobile phase consisting of methanol: chloroform: ammonium acetate (6:3:1 v/v/v). Densitometric analysis of metformin hydrochloride was carried out in the absorbance mode at 236 nm.The linearity was found to be in the concentration range of 100- 300 ng/spot. The % recovery of metformin hydrochloride was found to be 100.24-101.58%, indicating no interference from the excipients in the method. https://biomedpharmajournal.org/vol1no2/hptlc-method-for-estimation-of-metformin-hydrochloride/ HPTLC Metformin HCl validation