eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2015-01-22 1 2 433 436 496 article L.M.S. Rudrammaji 1 Sumasridhar 2 M.S. Dinesh 3 Veena. G. Sonole 1 Department of Biotechnology, Sri Jayachamarajendra College of Engineering, Mysore - 570 006 (India). Department of Biochemistry, Dayananda Sagar College of Dental Sciences, Kumaraswamy Layout, Bangalore - 560 078 (India). Department of Biotechnology, PES Institute of Technology, 100 Feet Ring Road, BSK III Stage, Bangalore - 560 085 (India). Cytotoxic effect of coelomic fluid of earthworm Eudrilus eugeniae was studied on Baby Hamster Kidney 21 (BHK21) Cells. Coelomic fluid from Eudrilus eugeniae was extracted by cold shock treatment. BHK21 cells were cultured according to standard cell culture method. BHK21 cells were treated with the various protein concentrations of coelomic fluid of Eudrilus eugeniae to study the cytotoxoc effect. The result revealed that the coelomic fluid of Eudrilus eugeniae induced cell death and the activity was concentration dependent, confirming the presence of cytotoxic molecule/s. https://biomedpharmajournal.org/vol1no2/cytotoxic-effect-of-coelomic-fluid-of-earthworm-eudrilus-eugeniae/ Coelomic fluid cytotoxic activity Eudrilus eugeniae