eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-09-30 19 3 2746 2754 10.13005/bpj/3531 73275 article Salma Amr 1 Lana Hamou 1 Waleed Ali 1 Jana Al Ahmad 1 Ramya Rathan 1 Miral Nagy Fahmy Salama 1 Department of Biomedical Science, Gulf Medical University, Ajman, UAE Placebo analgesia has been investigated across various pain models, but evidence on pressure pain modulation and gender related differences in young adults is limited. To evaluate the effects of placebo analgesia on pressure pain threshold (PPT) and pressure pain tolerance (PT), and to understand the gender-based differences in pain perception. A seven-month an uncontrolled pre–post quasi-experimental design with 161 people ages 18 to 25 was held at Gulf Medical, UAE. The pressure pain threshold (PPT) and pain tolerance (PT) on the thenar eminence of the dominant hand were measured using a digital pressure algometer. A placebo intervention was administered using a saline spray, falsely presented as an analgesic. Statistical analysis was done using the Wilcoxon Signed Ranks and the Mann-Whitney U tests. Post-placebo, both PPT and PT increased significantly across all participants (p < 0.001). Males consistently demonstrated higher values than females before and after the intervention. Notably, the placebo effect was more pronounced in PT values. While both sexes exhibited improvement, the gender-based differences in pain perception remained statistically significant. Placebo analgesia increases pain tolerance significantly, with notable gender-specific differences in pain perception. These findings underscore the potential of placebo mechanisms in pain modulation and support further research into non-pharmacological strategies for pain management. https://biomedpharmajournal.org/vol19no3/the-impact-of-placebo-analgesia-on-pressure-pain-threshold-and-tolerance-a-gender-based-experimental-study-in-young-adults/ Gender differences Pain Management Pain Perception Placebo Analgesia Pressure Pain Tolerance