eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-09-30 19 3 2768 2774 10.13005/bpj/3533 73135 article Dalia Hussien 1 Wala Eldin Elradi 1 Ashok Kumar Sah 2 Rabab Hassan Elshaikh Mahmoud 2 Faculty of Graduate Studies, Medical Laboratory Sciences Department, Al-Zaiem Al-Azhari University, Khartoum, Sudan College of Health and Applied Sciences, Medical Laboratory Sciences department, A’ Sharqiyah University, Ibra, Oman The oral contraceptive pills (OCPs) have been commonly used to regulate hormones and control conception in females. The use of OCPs causes marked changes in the coagulation pathway making them prone to venous thromboembolism (VTE). The D-dimer level has been found to be an important biomarker of fibrin turnover and a tool to interpret the possibility of VTE. This cross-sectional comparative study aims at assessing the effect of the OCPs on D-dimer levels and comparing them with the existing global evidence. The study was cross sectional in design; it was carried out in Khartoum, Sudan. It involved 200 women; 100 women in the case group were taking oral combined OCPs while the other 100 women who made up the control group were not using the OCPs. D-dimers concentration levels were measured using Ichroma™ II fluorescence immunoassay. The mean level of D-dimer was significantly elevated in the case group (418 ± 104.3 ng/mL) compared to the control group (223 ± 39.2 ng/mL; P < 0.001). In the case group, 62% of participants had a higher than clinical value of D-dimer (400 ng/mL), while only 5% of control group subjects showed a higher value. No significant relationship was detected between the level of D-dimer and age (P > 0.05), but the level showed a positive correlation with OCP period of use (P < 0.05). Women on OCP who are healthy show a state of continuous and physiological rise in their D-dimer concentration. This shows a condition of increased fibrin turnover. These findings suggest that OCP use may influence baseline D-dimer concentrations and could potentially affect the interpretation of D-dimer results in clinical practice. These findings should therefore be considered hypothesis-generating and warrant further prospective studies involving women with suspected VTE to determine whether OCP-specific reference ranges or diagnostic thresholds may be clinically useful. https://biomedpharmajournal.org/vol19no3/determination-of-d-dimer-levels-among-healthy-women-using-oral-contraceptives-a-cross-sectional-study-comparative-analysis/ Clinical laboratory analysis  D-dimer Hemostasis Oral contraceptives Venous thromboembolism Procoagulant state