eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-09-30 19 3 2563 2575 10.13005/bpj/3515 73440 article Made Dharmesti Wijaya 1 Anak Agung Gede Indraningrat 2 Wirdatun Nafisah 3 Nora Ertanti 4 Department of Pharmacology, Faculty of Medicine and Health Sciences, Warmadewa University, Jl Terompong No. 24, Denpasar-Bali, Indonesia Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Warmadewa University, Jl Terompong No. 24, Denpasar-Bali, Indonesia Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Surabaya, Jl. Ketintang Gedung D1, Surabaya-Jawa Timur, Indonesia Stem Cell Research and Development Center, Airlangga University, Jl. Mulyorejo Kampus C, Surabaya-Jawa Timur, Indonesia Breast cancer continues to impose a substantial global health burden, and natural products remain an important source of candidates for anticancer research. This study investigated the cytotoxic and apoptosis-associated effects of the ethanolic extract of Schefflera elliptica against T47D human breast cancer cells. Cytotoxicity was evaluated using the MTT assay, while apoptosis-associated responses were assessed by Annexin V-FITC/propidium iodide flow cytometry and caspase-3 and caspase-8 immunofluorescence. Metabolite profiling was performed using liquid chromatography–high-resolution mass spectrometry (LC-HRMS), followed by exploratory molecular docking of selected metabolites against the anti-apoptotic protein Bcl-2. The crude ethanolic extract exhibited moderate cytotoxic activity, with an IC50 of 339 μg/mL. Treatment increased the Annexin V-positive cell population, with significant increases observed at 160 and 320 μg/mL, and was accompanied by increased caspase-3 and caspase-8 immunofluorescence signals. LC-HRMS profiling yielded 39 putatively annotated metabolites, with choline, L-norleucine, and valine among the most abundant. Molecular docking predicted interactions of these metabolites within the Bcl-2 binding pocket, although their predicted binding affinities were weaker than those of venetoclax. Overall, the findings indicate that S. elliptica ethanolic extract exhibits moderate cytotoxic activity accompanied by apoptosis-associated cellular responses in T47D cells. The integrated biological, metabolomic, and computational findings provide a basis for further bioactivity-guided investigation of the active constituents and their underlying mechanisms. https://biomedpharmajournal.org/vol19no3/cytotoxic-and-apoptosis-associated-activity-of-schefflera-elliptica-blume-harms-ethanolic-extract-in-t47d-breast-cancer-cells-supported-by-lc-hrms-profiling-and-molecular-docking/ Apoptosis Breast Cancer Cytotoxicity LC-HRMS Molecular Docking Schefflera elliptica