eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-09-30 19 3 2675 2689 10.13005/bpj/3524 73479 article Windingoudi Rimwagna Christian Ouedraogo 1 Mathieu Nitiema 1 Boukaré Kabore 2 Souleymane Compaore 1 Aristide Traore 1 Moussa Ouedraogo 2 Département Médecine et Pharmacopée Traditionnelles – Pharmacie (MEPHATRA-PH), Institut de Recherche en Sciences de la Santé/Centre National de la Recherche Scientifique et Technologique (IRSS/CNRST), Ouagadougou, Burkina Faso Centre de Formation, de Recherche et d’Expertises en sciences du médicament (CEA-CFOREM), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso Institut National de la Jeunesse, de l’Education Physique et du Sport (INJEPS), Université d’Abomey-Calavi (UAC), Porto-Novo, Benin Vascular endothelium, in addition to functioning as a physical barrier, plays a crucial role in regulating blood pressure. Endothelial protective mechanisms offer an alternative way to prevent cardiovascular risks, including hypertension. This study aimed to demonstrate the vasculoprotective and antihypertensive potential of the ethyl acetate fraction of Phaseolus vulgaris (EPV) pods in mice. Naval Medical Research Institute (NMRI) mice were randomized, and hypertension was induced by oral administration of LNAME (Lω- Nitro Arginine Methyl Ester). Subsequently, EPV and captopril were administered to the hypertensive mice. Physical parameters, blood pressure components, and biochemical conditions were measured. Vascular protection was assessed by measuring NO-dependent vasorelaxation of the aortic ring in the study mice. Phytochemical analysis of EPV was performed by high-performance liquid chromatography-mass spectrometry. Captopril and EPV significantly reduced blood pressure in LNAME-induced hypertensive mice, with the most effective doses of 5 mg/kg captopril and 20 mg/kg EPV. Furthermore, two-way ANOVA with Bonferroni post-tests showed that mice treated with EPV responded better in aortic rings, with strong vasorelaxation in the presence of ACh (acetylcholine) and SNP (sodium nitroprusside), compared with mice treated with LNAME alone. The weight variation and biochemical parameters in high-tension mice improved significantly with doses of captopril and EPV. Molecules are known to have beneficial effects on cardiovascular health, most of which are chlorogenic acid, quercetin glucoside, and p-coumaroylquinic acid. This investigation showed that the EPV had antihypertensive and cardiovascular effects in LNAME hypertensive mice, as well as a protective effect on the vascular endothelium. https://biomedpharmajournal.org/vol19no3/antihypertensive-and-vascular-endothelium-protection-effect-of-the-ethyl-acetate-fraction-of-phaseolus-vulgaris-pods-in-l-name-induced-hypertensive-mice/ Antihypertensive mice Blood pressure LC-MS profile LNAME Phaseolus vulgaris Vascular endothelium