eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-07-17 19 3 72756 article Kadagoni Pravalika 1 Rakam Gopi Krishna 2 Suvendu Saha 3 Chavana Pranaswi 1 Jilla Bhavana 1 Gaddameedi Praneeth Goud 1 Department of Pharmaceutical Analysis, Marri Laxman Reddy Institute of Pharmacy, Dundigal (V and M), Hyderabad, Telangana, India Department of Pharmaceutical Chemistry, Marri Laxman Reddy Institute of Pharmacy, Dundigal (V and M), Hyderabad, Telangana, India Department of Pharmacology, Marri Laxman Reddy Institute of Pharmacy, Dundigal (V and M), Hyderabad, Telangana, India The recommended model is suitable for the quantifiable estimation of sitagliptin and also saxagliptin according to experimental findings. The present research work aims to develop RP-HPLC method for the quantification of sitagliptin and saxagliptin simultaneously in bulk dose form and validate according to ICH guidelines. Chromatographic analysis was carried out using a Discovery C18 column (4.6 x 150 mm, 5 µm). "The optimized mobile phase, comprising formic acid buffer, acetonitrile, and methanol (80:10:10 v/v/v), was delivered at 1.0 mL/min at 30°C. Under these conditions, saxagliptin and sitagliptin were eluted consistently at retention times of 2.212 and 2.642 minutes, respectively, with well-resolved and sharp peaks." Saxagliptin's percentage RSD was 0.5, while Sitagliptin's was 1.1. The % recovery for Saxagliptin is 99.61% and for Sitagliptin, it was 100.20% correspondingly. The LOD besides LOQ, derived values from the equations of regression, were 0.10 and 0.31 for saxagliptin, and 1.69 and 5.13 for sitagliptin respectively. The regression equation for Saxagliptin was y = 12784x + 1088.7, and for Sitagliptin, it was y = 12398x + 11053. The declined retention times and run time make this method determined to be straightforward and reasonably priced, appropriate for reliable industrial tested for control of quality. Using the requirements set forth by the International Council for Harmonization, it was determined that the suggested technique was straightforward, exact, accurate and validated. https://biomedpharmajournal.org/vol19no3/an-optimized-and-validated-rp-hplc-method-for-contemporaneous-estimation-of-sitagliptin-and-saxagliptin-in-bulk-and-tablet-dosage-forms/ Quality control Regression RP-HPLC Saxagliptin Sitagliptin Temperature