eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-06-29 19 2 72392 article Almurdi Almurdi 1 Rauza Sukma Rita 2 Dessy Arisanty 2 Zelly Dia Rofinda 1 Department of Clinical Pathology, Faculty of Medicine, Andalas University, Padang, Indonesia Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia Vascular endothelial growth factor (VEGF) is recognized as a cytokine that is generated by endothelial cells. It contributes to inflammation and increased vascular permeability.  VEGF has been correlated with disease severity, plasma leakage, and outcome in dengue. Serotype detection in dengue infections is crucial since secondary infections with heterologous serotypes can result in potentially fatal DHF and DSS. This study was conducted to determine the multiple dengue serotype infection by RT-qPCR and to identify VEGF levels in single and multiple serotype dengue infections. VEGF levels in this study were assessed by ELISA, whereas serotype was identified by molecular analysis. Data were analyzed with Student t-test. A total of 60 positive samples were analysed. Multiple-serotype dengue infections were detected in 20 samples (33.3%), while 40 samples (66.7%) had single-serotype infections. The mean VEGF level in multiple-serotype dengue infections (324.48 pg/mL) was not significantly different from that in single-serotype dengue infections (300.68 pg/mL), with a p-value of 0.709. Multiple-serotype dengue infections were identified in one-third of the study population. Patients with multiple-serotype infections showed higher mean VEGF levels than those with single-serotype infections; however, no statistically significant difference was observed. These findings suggest that VEGF may not differ substantially between single- and multiple-serotype dengue infections in the studied population. https://biomedpharmajournal.org/vol19no2/vascular-endothelial-growth-factor-levels-in-single-and-multiple-serotype-dengue-virus-infections/ Dengue virus Dengue hemorrhagic fever Endothelial permeability Multiple serotype infection RT-qPCR VEGF