eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-06-03 19 2 72089 article Naushad Edayadulla 1 Emil Jebaz Devasundar 2 Jeeva Rose Kanaka Raj 3 Chandraraj Shanmuga Sundari 4 Kirthika Ganesan 5 Department of Chemistry, Vel Tech Rangarajan Dr. Sagunthala R and D Institute of Science and Technology, Avadi, Chennai, India Department of Chemistry, Aalim Muhammed Salegh College of Engineering, Avadi, Chennai, India. Department of Chemistry, The Oxford College of Engineering, Bommanahalli, Bangalore – 560 068, Karnataka, India. The improper disposal of floral waste, particularly from religious and cultural activities, has become a significant environmental concern, contributing to pollution and the underutilization of valuable bioresources. The sustainable utilization of floral waste has received significant attention due to its pharmaceutical potential. This study utilizes floral waste as a valuable product due to its abundant availability in various locations. The phytochemicals were extracted from leftover flowers of Chrysanthemum morifolium and evaluated for their antibacterial properties. Preliminary phytochemical analysis confirms the presence of various secondary metabolites. The peaks at 268 nm and 331 nm in UV-visible spectroscopy indicate the presence of conjugated phytochemicals. Fourier transform infrared study revealed the many functional groups that contribute to biological characteristics. Gas chromatography–mass spectrometry analysis revealed 68 bioactive compounds present in the flower and five compounds as major phytoconstituents based on area percentage. The antibacterial activity of the extract was evaluated against Escherichia coli and Staphylococcus aureus via the agar diffusion method, revealing significant inhibitory effects on both strains. To analyse the interaction between the protein and the bioactive compounds, in silico computational studies were performed for the five major constituents against bacterial target proteins (PDB IDs: 6G9S and 4URN). The docking results indicate the binding affinity and interaction between the ligand and the amino acid active site, validating the observed antibacterial efficacy. https://biomedpharmajournal.org/vol19no2/experimental-and-molecular-docking-analysis-of-phytochemicals-from-chrysanthemum-morifolium-for-antibacterial-applications/ Antibacterial Activity Chrysanthemum morifolium Floral Waste Molecular Docking Phytochemical Analysis