eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-06-29 19 2 72421 article Ibrohim Rakhmonovich Askarov 1 Yusupjon Tojimamatovich Isayev 1 Sanjar Ashiraliyevich Rustamov 1 Baxrom Abduxalimovich Mirzaabdullayev 1 Dostonbek Usmonjon o‘g‘li Egamberdiyev 1 Lazizbek Makhmudov 2 Department of Chemistry, Andijan State University, Andijan, Uzbekistan Department of Plant Cytoprotectors and Pharmacology, A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan Department of Natural Sciences, Guliston State University, Syrdarya, Uzbekistan Pulmaklin is a flavonoid-rich supplement derived from traditional medicinal plants. This study evaluated the anti-inflammatory efficacy of the biologically active supplement "Pulmaklin" under experimental conditions. The effects of the preparation in syrup form at doses of 50 and 100 mg/kg were investigated, and the highest anti-exudative activity (AEA) was observed at 50 mg/kg, with a 54.3% reduction in edema development. Based on the results, the optimal dose of Pulmaklin was scientifically substantiated. Molecular-level analysis demonstrated that flavonoids, particularly the glycoside form rutin, exhibit the highest affinity toward the COX-2 enzyme due to their ability to form multiple interaction points. In addition, flavonoids such as rutin and quercetin showed effective modulation of IL-6 cytokine activity and MAPK signaling pathways, contributing to their anti-inflammatory effects. The findings confirm that flavonoids and their derivatives are promising natural bioactive compounds with significant anti-inflammatory potential. https://biomedpharmajournal.org/vol19no2/determination-of-the-anti-inflammatory-effective-dose-of-the-biologically-active-compound-pulmaklin/ Anti-exudative activity Biologically active supplement COX-2 Flavonoids IL-6 Inflammation MAPK Molecular docking Natural bioactive compounds Pulmaklin Quercetin Rutin