eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-05-19 19 2 71866 article Аriunzaya Lkhagvaa 1 Jambaninj Dambiinyam 1 Anujin Tseveenjav 2 Anand Altankhuyag 3 Khongor Bat-Erdene 4 Maralgua Avirmed 1 School of Pharmacy, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia Department of Pathology and Forensic Medicine, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia. Department of Immunology, School of Bio-Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia. Institute of Physics and Technology of the Mongolian Academy of Sciences, Ulaanbaatar, Mongolia Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia Herbal products are considered important medicinal sources in both traditional medicine and modern pharmacology. Our study examined the lung-protective and anti-inflammatory properties of the herbal formulation in alipopolysaccharide (LPS)induced model of acute lung injury. Non-cytotoxic concentrations in cells were evaluated in vitrousing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and analysis of nitric oxide (NO) production. In our invivo study, acute lung inflammation was induced in experimental animals by LPS. Treatment with the herbal formulation notably decreased NO production, increased superoxide dismutase (SOD) levels, and reduced the protein levels of tumor necrosis factor-alpha (TNF-α) and high-mobility group box 1 (HMGB1). Furthermore, histopathological studies confirmed its protective effects against LPS-induced lung inflammation. Molecular docking revealed that glycyrrhizic acid and inulin interact strongly with TNF-α and HMGB1 proteins, supporting their potential to mediate the observed anti-inflammatory effects.In conclusion, the herbal formulation demonstrates anti-inflammatory and lung-protective properties, suggesting its potential efficacy in mitigating LPS-induced acute lung injury. https://biomedpharmajournal.org/vol19no2/anti-inflammatory-and-lung-protective-effect-of-the-herbal-formulation-on-lps-induced-acute-lung-injury-model/ Anti-Inflammatory In silico In vitro LPS-induced acute lung injury Lung protective