eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-03-20 19 1 491 502 10.13005/bpj/3368 70431 article Sangeetha Raja 1 Satyajit Mohapatra 2 Kalaivani Amitkumar 3 Kasthuri Natarajan 4 Jamuna Rani 1 Alwin Devasagayam 5 Department of Pharmacology, SRM Medical College Hospital and Research Centre, SRMIST, SRM University, Kattankulathur, Chengalpattu District, Chennai, Tamil Nadu, India Department of Pharmacology, All India Institute of Medical Sciences, Changsari, Kamrup District, Guwahati, Assam, India Department of Pathology, SRM Medical College Hospital and Research Centre, SRMIST, SRM University, Kattankulathur, Chengalpattu District, Chennai, Tamil Nadu, India Department of Biomedical sciences, Saveetha college of Allied Health Sciences, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Thandalam, Chennai, Tamil Nadu, India Department of Central Animal House, SRM Medical College Hospital and Research Centre, SRMIST, SRM University, Kattankulathur, Chengalpattu District, Chennai, Tamil Nadu, India Department of Pharmacology, Sree Balaji Medical College and Research Institute, Chrompet, Chennai, Tamil Nadu, India Abnormal lipids level leads to hyperlipidemia. Safe and effective therapy is essential for management of hyperlipidemia.   Vetiveria zizanioides (VZ), commonly known as vetiver or khus, in traditional medicine shows promising treatment in hyperlipidemia without compromising reproductive health. Aim of this study is to assess antihyperlipidemic effect of aqueous and ethanolic extract from Vetiveria zizanioides in high-fat diet-induced Wistar Albino rats. The aqueous and ethanolic extract of Vetiveria zizanioides was administered orally for 8 weeks at dosage of 300/600mg/kg of wistar albino rats. Body weight, histology and biochemical assessments of the rats were analysed. In-silico evaluation of the Vetiveria zizanioides was done prior to the animal study for the better understanding of the herb.  Body weight was decreased 50% in aqueous extract VZ-treated group compared to control group. Biochemical assessments indicate significant decrease in total and LDL cholesterol, triglycerides levels and improvement in HDL levels in high fat diet (HFD) and combination with simvastatin (HFD+SIM) groups (P<0.000). Histology revealed reduction in necrosis and edema while retaining normal spermatogenesis in VZ-treated HFD rats. In in-silico analysis, thirty-six targets were identified between the hypolipidemia and rat testis. Molecular dynamics simulations confirmed the dynamic stability interaction of globulol with highest binding affinity for target proteins 7-dehydrocholesterol reductase (DHCR7) and squalene synthase (FDFT1), with binding energies of -6.8 and -8.6 kcal/mol, respectively and thus ability to modify the protein targets.  In conclusion, the in-silico and in-vivo analysis confirmed the antihyperlipidemic activity of Vetiveria zizanioides. https://biomedpharmajournal.org/vol19no1/proliferative-and-protective-effects-of-vetiveria-zizanioideson-obesity-and-male-fertility-in-wistar-albino-rats/ Cholesterol Hyperlipidemia In-silico analysis Testis histology Vetiveria zizanioides  Wistar rat