LC-MS Based Metabolomics, In vitro and In silico Approaches to Characterize Possible Inhibitors of Alpha-glucosidase and Protein Glycation from Premna serratifolia L: Implications in Controlling Diabetes

eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-03-20 19 1 264 281 10.13005/bpj/3351 70321 article LC-MS Based Metabolomics, In vitro and In silico Approaches to Characterize Possible Inhibitors of Alpha-glucosidase and Protein Glycation from Premna serratifolia L: Implications in Controlling Diabetes Sagar Bhayye 1 Rakhee Dangi 1 Sagar Datir 2 Department of Bioinformatics, Bharati Vidyapeeth (Deemed to be University), Pune, India. Department of Cell and Molecular Biology, Bharati Vidyapeeth (Deemed to be University), Pune, India. Department of Plant taxonomy, Naoroji Godrej Centre for Plant Research, Pune, India. Ethnobotanically, Premna serratifolia has been used in folk medicine to treat a number of aliments including diabetes. However, there is no data on its antiglycation activity. The present study evaluated the alpha-glucosidase inhibition potential, antiglycation, cell protective and antioxidant potential of crude total methanol and fractionated hexane, ethyl acetate and methanol extracts prepared from leaves and stem bark of P. serratifolia. The stem ethyl acetate extract (S-EA) had the highest alpha-glucosidase inhibition potential, total phenolic and flavonoid content. The S-EA extract also displayed highest total antioxidant activity and highest reducing power. The total methanol extract of leaves (L-TM) displayed maximum free radical scavenging activity evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS). All aqueous extracts of leaves and stem exerted noticeable antiglycation potential evaluated using multistage glycation markers (fructosamines, protein carbonyls, thiols and β aggregation) with reduction in carbonyl levels and increase in free thiol groups (R=0.890; p<0.05) well correlated. However, the S-EA extract was more efficient in protecting erythrocytes from oxidative damage induced by glycated albumin. LC-MS-based metabolomic profiling of the S-EA extract, combined with in silico molecular docking studies, identified six compounds—6-hydroxy salvinolone, 10-O-trans-p-coumaroylcatalpol (10OCC), 10-O-trans-p-methoxycinnamoylcatalpol, Premnacorymboside B, 4-hydroxy-E-globularinin (B), and Premnaodoroside B—as putative novel inhibitors of α-glucosidase. These compounds warrant further purification and experimental validation to confirm their inhibitory activity. https://biomedpharmajournal.org/vol19no1/lc-ms-based-metabolomics-in-vitro-and-in-silico-approaches-to-characterize-possible-inhibitors-of-alpha-glucosidase-and-protein-glycation-from-premna-serratifolia-l-implications-in-controlling-diabe/ Anti-diabetic Anti-glycation Docking Iridoid-glycosides Stem