eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-03-20 19 1 330 347 10.13005/bpj/3356 69908 article Renuka Saravanan 1 Sivakumar Ramalingam 1 Department of Chemistry and Biosciences, SASTRA Deemed to be University, Srinivasa Ramanujan Centre, Kumbakonam, Tamil Nadu, India. In nanomedicine, there has been a lot of interest in creating sustainable and environmentally friendly processes that generate nanoparticles. In the current study, an environmentally friendly and economical method was used to produce silver nanoparticles (AgNPs) using Pergularia daemia (Forssk.) Chiov., aqueous leaf extract. The manufactured AgNPs were assessed using Fourier Transform Infrared Spectroscopy (FTIR), Zeta potential analysis, UV-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), and a particle size analyzer. AgNP creation was validated by the unique surface plasmon resonance (SPR) peak seen at 442 nm. SEM analysis revealed a spherical morphology, while FTIR indicated the occurrence of functional groups responsible for nanoparticle stabilization. The generated AgNPs had a normal particle size of 137.5 nm and a zeta potential of -20.7 mV, indicating good colloidal stability. The anticancer efficacy of AgNPs was evaluated against HepG2 liver cancer cells using MTT, LDH, and Trypan Blue dye exclusion assays, demonstrating significant cytotoxic activity. Additionally, the antioxidant activity of the nanoparticles suggests their capability to moderate oxidative stress. This study highlights the promise of Pergularia daemia (Forssk.) Chiov., as a natural reducing and stabilizing agent for AgNP synthesis, offering a promising avenue for the development of plant-based nanotherapeutics. Further investigations into mechanistic pathways and in vivo studies could pave the way for their clinical applications in cancer treatment. https://biomedpharmajournal.org/vol19no1/green-fabrication-of-pergularia-daemia-mediated-silver-nanoparticles-for-antioxidant-and-hepatocellular-carcinoma-applications/ Anticancer activity HepG2 cells Nanomedicine Pergularia daemia and Silver nanoparticles