eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2026-03-20 19 1 592 606 10.13005/bpj/3378 71086 article Lobna Saad Mohammed Abd Elmeged 1 Ali Khalaf Ahmed Albaggar 3 Department of Nutrition, Applied College, AL-Baha University, Al-Makhwa, Saudi Arabia Department of Nutrition and Food Sciences, Faculty of Home Economics, Menoufia University, Shibin el Kom, Menofia Governorate, Egypt 2Department of Public Health, Faculty of Applied Medical Sciences, AL-Baha University, Al-Baha, Saudi Arabia Plant phytochemicals are widely recognized for their hepatoprotective and antioxidant properties, playing an important role in protecting the liver from chemically induced oxidative damage. This study aimed to evaluate the protective effects of dietary green fenugreek and black mulberry against carbon tetrachloride (CCl₄)-induced oxidative stress in rats. Thirty male rats were divided into five groups: an untreated control, a CCl₄-exposed control, and three groups receiving diets supplemented with green fenugreek, black mulberry, or a combination of both. The effects of supplementation were assessed on antioxidant enzyme activity, liver and kidney function, and lipid profiles. Additionally, histopathological examination of the liver was conducted to determine tissue-level protection. The results showed that plant supplementation significantly enhanced antioxidant enzyme activity, normalized liver and kidney function, and restored lipid balance compared with CCl₄-treated controls. Histological analysis confirmed better preservation of liver architecture, with the combined supplementation of green fenugreek and black mulberry providing the most pronounced protective effect. These findings indicate that dietary green fenugreek and black mulberry effectively counteract oxidative stress, improve organ function, and may offer synergistic benefits when used together, supporting their potential as functional dietary interventions for liver health. https://biomedpharmajournal.org/vol19no1/effects-of-raw-materials-from-green-fenugreek-and-black-mulberry-on-oxidative-stress-biomarkers-induced-by-carbon-tetrachloride-toxicity-in-experimental-rats/ Carbon tetrachloride-induced hepatotoxicity Metabolic homeostasis Morus nigra oxidative stress Trigonella foenum-graecum