eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-12-30 18 4 2880 2890 10.13005/bpj/3302 69643 article Sri Wahjuni 1 I Gusti Agung Gede Bawa 1 Anak Agung Istri Agung Mayun Laksmiwati 1 Ida Ayu Raka Astiti Asih 1 Ni Made Nonik Sawitri 1 Department of Chemistry Faculty of Mathematics and Natural Sciences Udayana University, Jalan Raya Kampus Unud, Jimbaran, Badung, Bali, Indonesia The red galangal rhizomes (Alpinia purpurata K. Schum) plant has natural product potential depending on the amount of active compound extracted and the extraction method. This study aimed to determine the effect of the administration of ethanol red galanga rhizomes of suplementation to histopathological changes in breast cancer and identified the coumpound content of red galangal rhizomes (Alpinia purpurata K. Schum). Use of phytochemical screening methods, identification using LC-MS/MS instruments, histopathological examination of breast glandular tissue, benzo(a)pyrene,Vacum rotary evaporator, set of tools LC-MS/MS AC QUITY UPLC®H- Class System (Waters, USA). The red galangal rhizome (Alpinia purpurata K.Schum) was obtained from Badung Regency, Bali, Indonesia, and determined at the LIPI-UPT Center for Plant Conservation of the Bali “Eka Karya” Botanical Garden. The red galanga rhizomes reported that the higher the ethanol concentration. Red galanga rhizomes contain perhexiline as an anti-breast cancer agent. Research shows growing evidence concerning toxic effects induced by benzo(a)pyrene substance. This xenobiotic is metabolized by cytochrome P450 (CYP P450) to carcinogenic metabolite: 7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), which creates DNA adducts, causing mutations and malignant transformations. CYP P450 is strongly involved in B[a]P metabolism, and it is simultaneously expressed as a result of the association of B[a]P with aromatic hydrocarbon receptor (AhR), playing an essential role in the carcinogenic potential of various xenobiotics. In turn, polymorphism of CYP P450 genes determines the sensitivity of the organism to B[a]P. Ethanol extract galangal rhizomes contains alkaloid, flavonoid, polifenol, steroid, triterpenoid and saponin compounds. Contained Perhexiline has the anticancer activity of red galanga rhizomes with benzo(a)pyrene induction at various doses the ethanol extract of red galangal rhizomes had an effect in improving the histopathological picture of mouse breast cancer cells at doses of 70 mg/kgBW/day and 90 mg/kgBW/day. https://biomedpharmajournal.org/vol18no4/phytochemical-screening-and-evaluation-of-the-effect-of-ethanol-extract-red-galanga-rhizomes-extract-for-histopathology-of-breast-cancer-cells-in-mice/ Anticancer Breast cancer Benzo(a)pyrene Histopathology Red galanga rhizomes