eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-12-30 18 4 3186 3203 10.13005/bpj/3327 69267 article Iroda Ganieva 1 Ilyos Xudoynazarov 1 Alisher Khaitbaev 1 Muzaffar Shokirov 1 Mirjalol Jalilov 1 Dilshod Mansurov 1 Department of Natural compounds and applied Chemistry, National University of Uzbekistan, Tashkent, Uzbekistan Rosmarinus officinalis L. (rosemary) is a medicinal and aromatic plant traditionally valued for its antioxidant, antimicrobial, and anti-inflammatory activities, largely due to its terpenoid-rich essential oils. In this study, 1,8-cineole was successfully isolated in pure form from rosemary cultivated in Uzbekistan using solvent extraction followed by vacuum fractional distillation, and its identity was confirmed by gas chromatography–mass spectrometry (GC–MS). Toxicological investigations demonstrated that oral administration of 1,8-cineole produced no adverse effects at doses of 200–400 mg/kg, while higher concentrations caused dose-dependent toxicity, with an LD₅₀ value of 680 mg/kg, classifying the compound as a low-toxic substance. Sub-chronic studies revealed no significant alterations in body weight, behavior, renal function, or hematological parameters, indicating good tolerance at repeated low doses. Complementary in silico ADMET predictions further confirmed favorable pharmacokinetic properties, including high gastrointestinal absorption, strong blood–brain barrier permeability, moderate clearance, and compliance with major drug-likeness rules, with minimal predicted toxicity. Additionally, molecular docking analyses demonstrated that 1,8-cineole interacts with key inflammatory mediators—COX-2 (−6.2 kcal/mol), IL-6 (−5.2 kcal/mol), MAPK (−5.3 kcal/mol), NF-κB (−5.2 kcal/mol), and TNF-α (−6.8 kcal/mol)—with the strongest affinities observed for COX-2 and TNF-α. These multitarget interactions suggest potential modulation of prostaglandin synthesis and cytokine signaling. Taken together, these findings highlight 1,8-cineole as a pharmacologically safe and promising bioactive compound, underscoring its potential for therapeutic and industrial applications. https://biomedpharmajournal.org/vol18no4/in-silico-anti-inflammatory-potential-admet-pharmacokinetics-and-isolation-of-18-cineole-from-rosmarinus-officinalis/ ADMET GC–MS pharmacokinetics Rosmarinus officinalis 1 8-cineole