eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-12-30 18 4 2783 2790 10.13005/bpj/3293 69258 article Mohamed Nabil Ibrahim 1 Eman Fawzy El Azab 1 Mohamed Eid Anwar 3 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences -Qurrayat, Jouf University, Al-Qurayyat, Saudi Arabia Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt. Department of Hematology, Shebeen El-Kom Hospital, El-Monofia, Egypt Herpes simplex virus type 2 (HSV-2) is still a common sexually transmitted disease (STD) linked to obstetric complications, such as miscarriage, premature birth, and herpes in newborns. Despite its prevalence worldwide, little information is known about pregnant Egyptian women. This cross-sectional hospital-based study aimed to evaluate the seroprevalence of HSV-2-specific IgG and IgM antibodies and examine their sociodemographic and clinical correlations by enrolling 185 pregnant Egyptian women aged 22 to 48. Serum samples were tested using the enzyme-linked immunosorbent assay (ELISA) technique. Statistical analyses were conducted using the chi-square test, with p < 0.05 considered statistically significant. 67% of the participants were positive for HSV-2 IgG, reflecting past infection, while 19.5% were positive for HSV-2 IgM, indicating recent or active infections. IgG positivity was strongly related to low socioeconomic status (p = 0.001), illiteracy (p = 0.001), and first-trimester pregnancy (p = 0.001). However, IgM positivity was clearly correlated with the gestational stage (p = 0.001). The high seroprevalence of HSV-2 among pregnant Egyptian women signposts the importance of both prevention strategies and routine prenatal screening. Improved healthcare accessibility and greater health literacy among women may play a critical role in reducing the adverse obstetric outcomes related to HSV-2 infection. https://biomedpharmajournal.org/vol18no4/hsv-2-seroprevalence-and-adverse-pregnancy-outcomes-among-egyptian-women-a-cross-sectional-study/ Herpes simplex virus type 2 (HSV-2) HSV-2 IgG HSV-2 IgM Maternal infection Pregnancy Prenatal screening Seroprevalence