eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-12-30 18 4 2608 2617 10.13005/bpj/3280 69580 article Ashwaq Taleb Kareem 1 Samer Khalid Ali 2 Department of Pharmacognosy, College of Pharmacy, Thi-Qar University, Thi-Qar, Iraq Pharmaceutic Department, College of Pharmacy, Al-Ayen Iraqi University, AUIQ, An Nasiriyah, Iraq Parsley leaves (Petroselinum crispum) biennial herb native to the Mediterranean has diverse bioactive phytochemicals, like flavonoids and phenolic acids that known for their potent antioxidant and cytotoxic properties. This study aimed to isolate two phenolic compounds, Apigenin and Syringic acid from ethyl acetate fraction of parsley leaves extract and evaluate their cytotoxic activity against A375 cell line compared to normal cells. Ultrasonication was used to extract the active compounds using 90% ethanol, and PHPLC was used to isolate the two phenolic compounds. FT-IR was used to categorize the active functional groups comprising the two isolated phenolic compounds. The results showed the isolation of syringic acid and apigenin, apigenin exhibited cytotoxic activity with IC₅₀% 40.8 μg /ml and Syringic acid exhibited cytotoxic activity with IC₅₀ values 54.5 μg /ml, Apigenin showing a stronger cytotoxic activity than Syringic acid. Both isolated phenolic compounds exhibited strong cytotoxic activity depend on increasing concentration of each compound, and Apigenin showing particularly high cytotoxic activity against A375 cell line. https://biomedpharmajournal.org/vol18no4/comparative-study-on-the-anticancer-potential-of-apigenin-and-syringic-acid-isolated-from-parsley-leaves-against-a375-cell-line/ A375cell line Apigenin Cancer Parsley Syringic acid