eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-12-30 18 4 2961 2974 10.13005/bpj/3309 68956 article Karthik Kadhiri Karunakar 1 Binoy Varghese Cheriyan 2 Bhavya E 1 Akshaya Murugathirumal 1 Abinaya Senthilkumar 1 Ragavendran Anandakumar 1 Department of Pharmacy Practice, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Chennai, TN, India Department of Pharmaceutical Chemistry, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Chennai , TN, India Nano biomedicine Lab, Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, TN, India Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, SRIHER, Chennai Oral cancer is an aggressive malignancy that typically originates in surrounding tissues and spreads to the cervical lymph nodes, with no effective chemotherapy currently available to prevent its progression. Recent studies have explored selenium-containing compounds as potential chemotherapeutic agents. This study used a biological approach to synthesis bioinspired selenium nanoparticles (SeNPs), which were then analyzed using Fourier Transform Infrared Spectroscopy (FTIR), Ultraviolet-Visible (UV-Vis) spectroscopy, Energy Dispersive X-ray Spectroscopy (EDX), and Scanning Electron Microscopy (SEM).Their anticancer and anti-inflammatory activities were evaluated against human oral squamous cell carcinoma (HOSCC) KB-1 cell lines using the egg albumin assay, MTT assay, and cytotoxicity assay at concentrations of 15 and 31 µg/ml. The SeNPs significantly reduced cell viability, induced apoptosis, and exhibited potent anti-inflammatory effects. Morphological alterations, such as cytoplasmic membrane blebbing and cell shrinkage, were observed under both inverted phase contrast and fluorescence microscopy, indicating apoptotic cell death. These findings demonstrate that bioinspired selenium nanoparticles effectively induce cytotoxicity and apoptosis in HOSCC KB-1 cells, highlighting their potential as promising agents for oral cancer therapy. https://biomedpharmajournal.org/vol18no4/bioinspired-selenium-nanoparticles-as-a-potential-anticancer-agent-in-oral-squamous-cell-carcinoma-apoptosis-induced-cytotoxicity/ Anticancer Agent Biological Synthesis Nanotechnology Oral Cancer Selenium Nanoparticles (SeNPs)