eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-12-30 18 4 2726 2740 10.13005/bpj/3289 68350 article Lakshmana Rao Atmakuri 1 Satya Sree Bandaru 2 Vasudha Dadi 3 Suresh Babu Madda 4 Vijaya Kumar Ghanta 5 Ramesh Alluri 6 Department of Pharmaceutical Analysis, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra Pradesh, India. Department of Pharmaceutical Chemistry, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra Pradesh, India. Department of Pharmaceutical Chemistry, Vignan Institute of Pharmaceutical Technology, Duvvada, Andhra Pradesh, India. School of Pharmacy, GIET University, Gunupur, Odisha, India. Department of Pharmacy Practice, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India. 6Department of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Telangana, India. A robust and repeatable RP-HPLC technique has been created for the concurrent measurement of Bupivacaine as well as Meloxicam in pharmaceutical formulation. The method underwent validation following applicable regulatory guidelines. The optimized chromatographic conditions included the use of a C18 Zorbax SB column (250x4.6 mm, 5 µ), an isocratic mobile phase comprising acetonitrile and a hexane sulfonic acid (2.5 pH adjusted with ortho-phosphoric acid) in a 60:40 volume ratio. The method operated at a rate of flow 1.0 ml/min, volume of 10 µl injection, UV detection set at 260 nm, as well as an ambient temperature of 25°C, with a total runtime of 5 min. Under these conditions, Bupivacaine eluted at 2.774 min and Meloxicam at 3.494 min. System suitability parameters, including, tailing factor, plate count as well as %RSD, were within acceptable limits. The method exhibited high precision, with %RSD values below 2%, and excellent linear correlation over a range of 50-300 µg/ml for Bupivacaine as well as 1.5-9 µg/ml for Meloxicam. Sensitivity analysis determined LOD and LOQ values of Bupivacaine 0.60 µg/ml and 2.00 µg/ml, for Meloxicam 0.018µg/ml and 0.060 µg/ml respectively. Forced degradation studies showed that the method could show stability under different stress conditions, with peroxide causing the highest degradation. The validated method was applied for assay of pharmaceutical formulation confirming compliance with label claim. https://biomedpharmajournal.org/vol18no4/a-novel-rp-hplc-method-for-the-quantitative-determination-of-bupivacaine-and-meloxicam-stability-indicating-approach/ Bupivacaine Forced degradation studies Meloxicam Method validation Pharmaceutical analysis RP-HPLC Stability-indicating method