eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-09-30 18 3 2239 2248 10.13005/bpj/3250 67903 article Ni Wayan Bogoriani 1 I Gusti Ayu Putu Eka Pratiwi 2 Ni Wayan Mirawati 3 Ida Ayu Raka Astiti Asih 4 Komang Tria Noviana Dewi 5 Dept. of Chemistry, University of Udayana, Bali, Indonesia Dept. of Doctor Education, University of Udayana, Bali, Indonesia Dept. of Chemistry, University of Udayana, Bali, Indonesia Dept. of Chemistry, University of Udayana, Bali, Indonesia Dept. of Pharmacy, University of Udayana, Bali, Indonesia Obesity represents a metabolic disorder associated with complications including hyperglycemia, dyslipidemia, and insulin resistance. The present study sought to characterize the bioactive compounds in red ginger extract through Liquid Chromatography–Mass Spectrometry (LC-MS) and to assess its therapeutic potential on body weight, glucose, triglyceride (TG), and free fatty acid (FFA) levels in Wistar rats with induced obesity. Female Wistar rats were induced to develop obesity with a high-fat intake until they reached a Lee index > 0.3. The experimental animals were allocated into four groups: a negative control, a positive control (obese untreated), and two treatment groups that received oral red ginger extract at doses of 100 mg/kg BW and 200 mg/kg BW for a duration of four weeks. The results demonstrated that both doses of red ginger extract significantly (p < 0.05) reduced weight, glucose, profile lipid (TG, and FFA) levels compared with the obese group. Specifically, glucose levels were reduced by 58.65% and 62.86% at 100 mg/kg BW and 200 mg/kg BW, respectively. Triglyceride levels decreased by 62.39% and 65.46%, while free fatty acid levels were reduced by 24.08% and 43.23%, respectively. LC-MS analysis identified 11 bioactive compounds in red ginger extract, including gallic acid, ar-curcumin, α-zingiberene, naringenin, 6-shogaol, kaempferol, catechin, 6-gingerol, quercetin, 8-gingerol, and rutin.   These findings indicate that the bioactive compounds in red ginger extract collectively provide a synergistic effect as a phytotherapeutic agent in the treatment of obesity and its metabolic complications. https://biomedpharmajournal.org/vol18no3/therapeutic-potential-of-red-ginger-zingiber-officinale-var-rubrum-bioactive-compounds-in-obesity-associated-metabolic-parameters-of-wistar-rats/ Blood Glucose Free Fatty Acids LC-MS Red Ginger Triglycerides