eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-09-30 18 3 2375 2393 10.13005/bpj/3263 67673 article Tasnia Binte Bari Kabbo 1 Pritesh Ranjan Dash 2 Adila Jahan Anika 3 Najiha Tarannum Timon 3 Tasnova Nowrin 4 Shammi Binte Bashar 2 Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh Department of Pharmacy, Primeasia University, Banani,Dhaka, Bangladesh Department of Pharmacy, University of Science and Technology Chittagong (USTC) Chittagong, Bangladesh Department of Management, Southern Utah University, Utah, United States Recent studies have focused on the pharmacological potentials of natural compounds as potent medicinal agents. Annona reticulata Linn. (Family: Annonaceae) has long been used as a remedy for a wide range of ailments by many tribes and ethnic groups worldwide. According to reports, the plant has a variety of medicinal properties, such as anthelmintic, antipyretic, antihyperglycemic, antiulcer, and antinociceptive activities. In addition to evaluating the plant's antioxidant, cytotoxic, and anti-cancer potentials, the current study used GC-MS analysis to identify the compounds causing these effects. Six established methodologies were utilized to evaluate the antioxidant potential. Good antioxidant properties were demonstrated by the extract in DPPH and nitric oxide radical scavenging tests, with IC₅₀ measurements of 96.15 µg/ml and 124.85 µg/ml. In both DPPH and nitric oxide radical scavenging tests ascorbic acid was used as standard; the observed IC₅₀ values for ascorbic acid in these two tests were 14.38 µg/ml and 67.31 μg/ml. In reducing power assay, the extract's antioxidant activity increased with increasing concentration. 136.4±6.35 mg/gm Ascorbic acid equivalents was the total antioxidant capacity value that was determined. The aqueous extract demonstrated total flavonoid content value of 89.15±0.4 mg/gm Quercetin equivalents and total phenol content value of 61.48±0.17 mg/gm Gallic acid equivalents.Good cytotoxic potential was demonstrated by the extract in the brine shrimp lethality test. This test yielded LC₅₀ value of 93.37 μg/ml for the aqueous leaf fraction and 1.63 μg/ml for standard vincristine sulphate; while the obtained LC₉₀ values for the aqueous leaf extract and standard vincristine sulphate were 516.65 μg/ml and 7.17 μg/ml.Additionally, the plant extract exhibited notable anti-cancer potential against the EAC cell line in in-vivo study. The groups of mice treated with 400 mg/kg dose of aqueous leaf fraction and 20 mg/kg dose of 5-FU demonstrated tumour weight and EAC cell number values of 3.00±0.38 g and 7.40±0.81; 5.08±0.35 g and 9.20±0.86, respectively. Therefore, the values obtained from aqueous extract were less than those of the standard medication 5-FU. https://biomedpharmajournal.org/vol18no3/evaluation-of-antioxidant-and-cytotoxic-potentials-against-the-eac-cell-line-of-aqueous-extract-of-annona-reticulata-linn-leaves/ Antioxidant effect Anti-cancer activity Bullock’s heart Cytotoxic effect Extract Leaves Tumor