eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-09-30 18 3 2102 2112 10.13005/bpj/3240 67878 article Chimedragchaa Chimedtseren 1 Dejidmaa Buyantogtokh 1 Myadagbadam Urtnasan 1 Anu Altangerel 1 Uuganbayar Baatartsogt 1 Erdenechimeg Chuluunbaatar 2 Department of Pharmacology, Research center, Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia Department of Chemistry and Technology, Research center, Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia Traditional Mongolian medicine encompasses various practices for treating hemorrhoids. One of the pharmaceutical formulations is Zidraga-6, which is composed of six active remedies. Aim of the study: The study sought to explore the phytochemical composition of Zidraga-6 and to thoughtfully evaluate its potential effects in a hemorrhoid model using rats. Methods: Thin-layer chromatography (TLC) was employed to identify biologically active compounds, and their concentrations were quantified using UV spectrophotometric methods. The experiment was conducted using a methodology established with a croton oil mixture. In a designated group, a hemorrhoid model was successfully induced in healthy animals using croton oil. Following a 24-hour period after the induction of the model, the Zidraga-6 herbal formulation was administered once daily over the course of eight days. Results: Using thin-layer chromatography (TLC), we  identified oleonalic acid, gallic acid, quercetin, capsaicin, and p-methoxycinnamate in the Zidraga-6 capsules. UV spectrophotometric analysis determined the content of bioactive compounds in Zidarga‑6 capsules to be 1.89 ± 0.19 % total triterpene saponins, 1.30 ± 0.12 % flavonoids, and 12.59 ± 0.68 % phenolic compounds. Using thin-layer chromatography (TLC), we  identified oleonalic acid, gallic acid, quercetin, capsaicin, and p-methoxycinnamate in the Zidraga-6 capsules. UV spectrophotometric analysis determined the content of bioactive compounds in Zidarga‑6 capsules to be 1.89 ± 0.19 % total triterpene saponins, 1.30 ± 0.12 % flavonoids, and 12.59 ± 0.68 % phenolic compounds. The results of this study indicated that all doses of Zidraga-6 capsules effectively reduced the inflammatory cytokine IL-6 in the rat hemorrhoid model. Furthermore, the levels of the cytokine IL-1β were significantly lowered with the 130 mg/kg and 325 mg/kg doses. Histopathological examination of the recto anal tissue demonstrated that the structural changes, inflammation, and necrosis were significantly diminished with the 130 mg/kg and 325 mg/kg doses of Zidraga-6 capsules when compared to the untreated hemorrhoid model. Conclusions: Zidraga‑6 capsules contain biologically active constituents such as flavonoids, phenolic compounds, and triterpene saponin.  Zidraga-6 has demonstrated efficacy in treating hemorrhoids by reducing pro-inflammatory cytokines and decreasing rectal damage. https://biomedpharmajournal.org/vol18no3/evaluation-of-anti-hemorrhoidal-activity-and-phytochemical-studyof-zidarga-6-capsule/ Croton oil Hemorrhoid model Inflammatory cytokine Total flavonoid Triterpene saponin