eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-09-30 18 3 1991 1999 10.13005/bpj/3231 67427 article Yusminah Hala 1 A Irma Suryani 1 Muhammad Junda 1 Hartono 1 Rachmawaty Muhtar 1 Andi Annisa 1 Department of Biology, Faculty of Mathematics and Natural Sciences, Makassar State University, Makassar, Indonesia Biosynthesis offers a safe and eco-friendly approach for producing nanoparticles using microorganisms or plant-derived reducing agents. This study explores the novelty of using endophytic bacteria isolated from the roots and stems of Lannea coromandelica, a medicinal plant with untapped potential in green nanotechnology, for the biosynthesis of silver nanoparticles (AgNPs). Four bacterial isolates were used Enterococcus (AM 2), Azotobacter (AM 5), Micrococcus (BM 2), and Enterococcus (BM 4), in reaction with 2–3 mM silver nitrate (AgNO₃). Characterization was performed using Particle Size Analyzer (PSA), UV-Visible Spectrophotometry (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). The synthesized AgNPs had particle sizes ranging from 60 to 105.9 nm. Notably, AgNPs synthesized using Azotobacter (AM 5) and Micrococcus (BM 2) showed optimal size and stability, with PI (Polydispersity Index) values of 0.029673 and 0.01835, respectively, indicating uniform particle distribution. UV-Vis spectra confirmed stability for up to five days. FTIR revealed the presence of functional groups O-H, C-N, and Ag-O, validating biosynthesis. SEM analysis demonstrated spherical to semi-spherical morphologies, with AM 5 exhibiting the most homogeneous structure. These findings highlight the promising role of L. coromandelica-derived endophytes in the eco-friendly synthesis of AgNPs for potential nanomedicine and antimicrobial applications. https://biomedpharmajournal.org/vol18no3/endophytic-bacteria-from-lannea-coromandelica-for-enhancing-silver-nanoparticle-biosynthesis/ Endophytic Bacteria Fourier Transform Infrared Lannea coromandelica Scanning Electron Microscopy Silver Nanoparticle