eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-06-30 18 2 1534 1544 10.13005/bpj/3191 66461 article Najwa Abdur Rashid 1 Mary Antony Praba 2 Mary Anne W.Cordero 1 Kavitha Ganesh 1 Raja El Hasnaoui-Saadani 1 Venkataramaniah Chinnappan 3 Department of Basic Medical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. Department of Anatomy, Sree Balaji Medical College, BIHER, Chromepet, Chennai, India. Department of Anatomy, Bhaarath Medical College, BIHER, Selaiyur, Chennai, India. With advancements in regenerative medicine based on human umbilical cord tissue derived mesenchymal stem cells (hUCT-MSCs) plays major role in clinical trials on tissue engineering and cell therapies to a variety of diseases like ischemia, diabetes, bone, cartilage and neurological disorders. Presently the gold standard supplement in culturing hUCT-MSC is foetal bovine serum (FBS), obtained in an unscrupulous way from the foetus of cow. Being a xenogeneic supplement, FBS increases the risk of zoonotic infection transmission and unfavourable immunological reactions in patients after transplantation. Also it is high on demand and with ethical issues. According to International Society for Cellular Therapy (ISCT) a supplement of culture media for hUCT-MSC must possess a few qualities like tri-lineage differentiation and must secrete certain cytokines with a zoonotic free environment and so called to find a human derived supplement alternate. Human platelet lysate (HPL) was considered a better supplement in culturing hUCT-MSCs but it can’t be stored in normal temperature nor reconstituted in time as it needed freezing thawing cycles. New researches saying that lyophilized human platelet lysate (LHPL) can be an effective alternate as it can be stored and reconstituted without zoonotic infections. So we have formulated this study so as to evaluate the potency of LHPL in fulfilling the ISCT criteria on proliferation, tri-lineage differentiation and on secretomes as a supplement in culturing hUCT-MSCs. https://biomedpharmajournal.org/vol18no2/tri-lineage-differentiation-and-secretome-analylsis-of-fbs-and-lhpl-supplemented-huct-mscs-a-comparative-evaluation-on-isct-criteria/ Alizarin red S Lyophilized human platelet lysate Mesenchymal stem cells Oil Red O Tri-lineage differentiation