eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-06-30 18 2 1381 1388 10.13005/bpj/3176 66042 article Fahima Aktar 1 Md. Naymor Rahaman 2 Hasina Akhter Simol 3 Mahfuza Afroz Soma 4 Md. Zakir Sultan 5 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh Department of Chemistry, University of Dhaka, Dhaka, Bangladesh Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh Cancer research graduate program, University of Nebraska Medical Center, Omaha, NE, USA. Seaweeds provide a range of crucial vitamins, among them water-soluble B complex vitamins such as thiamine (vitamin B1) and riboflavin (vitamin B2). The study focuses on the accurate quantification of thiamine and riboflavin in seaweed samples through an accurate and precise analytical process involving acid and enzymatic hydrolysis followed by HPLC analysis with a fluorescence detector. The applied methods were very accurate and precise as it performed recovery percentage about 99.06% for thiamine and 95.81% for riboflavin and RSD percentage less than 2% on average. Our findings reveal significant levels of thiamine and riboflavin present in the seaweeds which emphasis the nutritional value of seaweeds. Our study says that seaweeds contain up to 2.3 mg thiamin /100g and 0.9 mg riboflavin/100 g. The level of vitamins content varied with the variety of species and the environmental condition. Although with relatively lower riboflavin content, Seaweeds are very good source of vitamins. This study highlights the nutritional value of seaweeds which can be consider as powerhouse in nutritional context. Further exploration into other vitamins (water and fat soluble) and essential minerals would enhance our understanding of their nutritional significance. https://biomedpharmajournal.org/vol18no2/quantification-and-evaluation-of-thiamine-and-riboflavin-in-marine-resources-of-the-bay-of-bengal/ Fluorescence Detector HPLC Nutritional value Riboflavin Seaweeds Thiamine