eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-06-30 18 2 1085 1089 10.13005/bpj/3154 66568 article Jihan Hussein 1 Medical Biochemistry Department, Medical Research and Clinical Studies Institute, National Research Centre. Giza, Egypt. Chromatography is a process of separation that used to separate constituents in a mixture. This technique included two main phases: The stationary phase and the mobile phase. There are different types of chromatographic devices including gas chromatography, liquid chromatography, and ion exchange chromatography besides initial high performance liquid chromatography (HPLC). In this review we emphasis on the principles and applications of HPLC comparing with other devices of chromatography. Also we highlighted on the role of HPLC and its applications in different fields like medical, pharmaceutical, environmental and others. https://biomedpharmajournal.org/vol18no2/principles-and-applications-of-high-performance-liquid-chromatography-hplc-a-review/ Advancements Applications Column Technology HPLC Principle