eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-06-30 18 2 1499 1511 10.13005/bpj/3188 65716 article Jayaprakash Thirunavukarasu 1 Abdul Rahim Shajahan 1 Department of Chemistry, B S Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, Tamil nadu, India. N-phenyl Piperazine derivatives have increasingly been recognized for their profound medicinal properties. This study embarks on the synthesis and study of such derivatives, specifically focusing on their in vitro α-amylase inhibitory and anti-inflammatory potential. Employing in silico molecular docking strategies, we assessed the interactions of these derivatives with the α-amylase enzyme (1HNY.pdb). Compounds P6, P7, and P22 emerged with commendable docking scores  as -8.44, -8.37, and -8.49 kcal/mol, prompting their synthesis and assessment of in vitro α-amylase activity assessment. Among the studied compounds, P7 demonstrated robust inhibitory effects against both α- amylase and inflammation. Complementing the docking studies, this comprehensive investigation underscores the potential of N-phenyl Piperazine derivatives as potent bioactive molecules. https://biomedpharmajournal.org/vol18no2/antidiabetic-and-anti-inflammatory-properties-of-n-phenyl-piperazine-derivatives-through-in-vitro-evaluations-and-molecular-docking-studies/ Amylase inhibitor Anti-diabetic activity Anti-inflammatory N-phenyl piperazines