eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-06-30 18 2 1404 1412 10.13005/bpj/3179 65704 article Lakshmana Rao Atmakuri 1 Kurnool Mahammed Ismail 2 Vijaya Kumar Ghanta 3 Bhaskar Vallamkonda 4 Ramesh Alluri 5 Satya Venkata Sakuntala Mamidi 6 Department of Pharmaceutical Analysis, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra Pradesh, India. Department of Pharmaceutical Analysis, Nizam Institute of Pharmacy, Deshmukhi, Telangana, India. Department of Pharmacy Practice, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India. 4Department of Pharmaceutical Sciences, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, India Department of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Telangana, India. Department of Pharmacy, Government Polytechnic for Women, Kakinada, Andhra Pradesh, India. Current study was intended to establish and validate an advanced, novel, stable, simple and systematic analytical procedure to determine Bisoprolol & Cilnidipine in pure drug & tablet by Reversed-Phase HPLC method. The partition of analytes was attained with a combination of reverse phase C18 column (Waters X Terra 150x4.6mm; 3.5µm) and a mobile phase made up of 60% acetonitrile and 40% 0.1% trifluoroacetic acid is utilized, at a flow rate of 1mL/min over an 8minute, isocratic flow and at 225nm isobestic point. With the best acceptable resolution the peaks of Bisoprolol and Cilnidipine was eluted at 3.129min and 6.925min respectively. The linearities of the analytes especially with this proposed method were 2.52 to 37.80µg/ml & 5.00 to 75.00µg/ml for Bisoprolol and Cilnidipine respectively. For the proposed method, all the validation parameters were tested as indicated by ICH guidelines. The %RSD values for system precision was 0.41% for BISO and 0.14% for CILN, %RSD values for method precision was 0.4% for BISO and 0.3% for CILN and %RSD values for intermediate precision was 0.4% for BISO and 0.3% for CILN respectively. As per the validation report, the results of induced degradation studies, stability, precision, accuracy and the remaining validation parameters, however, were all excellent and within the acceptance criteria. The comparative study results reveal that the established process was economic, fast, suitable, sensitive and stable technique for determination of Bisoprolol & Cilnidipine in pure & tablet formulation. https://biomedpharmajournal.org/vol18no2/a-novel-analytical-method-for-simultaneous-quantification-of-bisoprolol-and-cilnidipine-by-reversed-phase-hplc-in-pure-and-tablet-dosage-form/ Bisoprolol Cilnidipine RP-HPLC Stability Validation