eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 947 956 10.13005/bpj/3143 64706 article Hayder Abbas 1 Zainab Zakaraya 2 Husni Farah 1 Sana Audeh 3 Mohammad Aladwan 4 Fouad El-shehabi 1 Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University/ Amman, Jordan Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Jordan. Orthopedics department, Medway Maritime Hospital, United Kingdom. Department of Medical Engineering, Faculty of Engineering, Al-Ahliyya Amman University/ Amman, Jordan. 4 Department of Biomedical Engineering, Faculty of engineering, Al-Ahliyya Amman University/ Amman, Jordan Hemolysis is a prevalent source of discrepancies in blood sampling. This study, involving 68 volunteers, systematically assessed the impact of hemolysis on Alkaline phosphatase (ALP) and Ceruloplasmin (Cp) levels, as well as the interplay of Zinc (Zn), Magnesium (Mg), and Copper (Cu) ions.  Blood samples (n=68) were subjected to mechanical trauma to produce varying degrees of hemolysis - mild, moderate, and severe. After hemolysis occurred, the ALP activities were measured and recorded as 74.94±15.98 IU/L, 63.47±18.51 IU/L, and 50.29±17.23 IU/L respectively, against 89.47±18.13 IU/L in non-hemolyzed samples. Cp levels were found to decrease in a manner dependent on the degree of hemolysis to 38.53±5.14 mg/dL, 36.29±4.85 mg/dL, and 34.12±5.08 mg/dL from an initial 41.06±6.13 mg/dL in non-hemolyzed samples. The ion concentrations (Mg, Zn, Cu) in hemolyzed blood samples exhibited a direct correlation with the extent of hemolysis. When specific concentrations of these ions equivalent to those measured during severe blood hemolysis were introduced to non-hemolyzed samples, the activity of the ALP enzyme was notably reduced (p<0.001). The concentration of Cp decreased upon the addition of Mg and Zn alone. These findings highlight the crucial role of hemolysis and metal ions in influencing the level of  ALP and Cp. https://biomedpharmajournal.org/vol18no1/the-effect-of-zinc-magnesium-and-copper-ions-on-the-activity-of-alkaline-phosphatase-enzyme-and-ceruloplasmin-protein-in-hemolyzed-blood-samples/ Alkaline phosphatase Ceruloplasmin Copper Hemolysis Magnesium Zinc